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Transcriptome profiling reveals the response process of tomato carrying Cf-19 and Cladosporium fulvum interaction.
BMC Plant Biology ( IF 5.3 ) Pub Date : 2019-12-19 , DOI: 10.1186/s12870-019-2150-y
Tingting Zhao 1 , Wenhong Liu 1 , Zhentong Zhao 1 , Huanhuan Yang 1 , Yufang Bao 1 , Dongye Zhang 1 , Ziyu Wang 1 , Jingbin Jiang 1 , Ying Xu 1 , He Zhang 1 , Jingfu Li 1 , Qingshan Chen 1, 2 , Xiangyang Xu 1, 3
Affiliation  

BACKGROUND During tomato cultivation, tomato leaf mould is a common disease caused by Cladosporium fulvum (C. fulvum). By encoding Cf proteins, which can recognize corresponding AVR proteins produced by C. fulvum, Cf genes provide resistance to C. fulvum, and the resistance response patterns mediated by different Cf genes are not identical. Plants carrying the Cf-19 gene show effective resistance to C. fulvum in the field and can be used as new resistant materials in breeding. In this study, to identify key regulatory genes related to resistance and to understand the resistance response process in tomato plants carrying Cf-19, RNA sequencing (RNA-seq) was used to analyse the differences between the response of resistant plants (CGN18423, carrying the Cf-19 gene) and susceptible plants (Moneymaker (MM), carrying the Cf-0 gene) at 0, 7 and 20 days after inoculation (dai). RESULTS A total of 418 differentially expressed genes (DEGs) were identified specifically in the CGN18423 response process. Gene Ontology (GO) analysis revealed that GO terms including "plasma membrane (GO_Component)", "histidine decarboxylase activity (GO_Function)", and "carboxylic acid metabolic process (GO_Process)", as well as other 10 GO terms, were significantly enriched. The "plant hormone signal transduction" pathway, which was unique to CGN18423 in the 0-7 dai comparison, was identified. Moreover, ten key regulatory points were screened from the "plant hormone signal transduction" pathway and the "plant pathogen interaction" pathway. Hormone content measurements revealed that the salicylic acid (SA) contents increased and peaked at 7 dai, after which the contents deceased and reached minimum values in both CGN18423 and MM plants at 20 dai. The jasmonic acid (JA) content increased to a very high level at 7 dai but then decreased to nearly the initial level at 20 dai in CGN18423, while it continued to increase slightly during the whole process from 0 to 20 dai in MM. CONCLUSIONS The initial responses are very different between the resistant and susceptible plants. The "plant hormone signal transduction" pathway is important for the formation of Cf-19-mediated immunity. In addition, both JA and SA play roles in regulating the Cf-19-dependent resistance response.

中文翻译:

转录组谱分析揭示了番茄携带Cf-19和黄萎病菌相互作用的响应过程。

背景技术在番茄栽培过程中,番茄叶霉病是由富叶隐孢子虫(C. fulvum)引起的常见病。通过编码Cf蛋白,它可以识别由黄花假丝酵母产生的相应AVR蛋白,Cf基因提供了对黄花假丝酵母的抗性,并且由不同的Cf基因介导的抗性应答模式并不相同。携带Cf-19基因的植物在田间表现出对黄腐念珠菌的有效抗性,可以用作育种中的新抗性材料。在这项研究中,为了鉴定与抗性相关的关键调控基因并了解携带Cf-19的番茄植物的抗性应答过程,使用RNA测序(RNA-seq)分析了抗性植物(CGN18423)携带的Cf-19之间的差异Cf-19基因)和易感植物(Moneymaker(MM),带有Cf-0基因)在0,接种后第7天和20天(每日)。结果在CGN18423应答过程中特异性鉴定出总共418个差异表达基因(DEG)。基因本体论(GO)分析显示,GO术语(包括“质膜(GO_Component)”,“组氨酸脱羧酶活性(GO_Function)”和“羧酸代谢过程(GO_Process)”以及其他10个GO术语都显着丰富。鉴定了“植物激素信号转导”途径,其在0-7 dai比较中是CGN18423独有的。此外,从“植物激素信号转导”途径和“植物病原体相互作用”途径中筛选出十个关键调控点。激素含量测量表明,水杨酸(SA)含量增加并在第7天达到峰值,此后,CGN18423和MM工厂在第20天的含量下降并达到最小值。茉莉酸(JA)含量在第7天增加到非常高的水平,但随后在CGN18423中降低到第20天的初始水平,而在整个过程中从MM的0到20 dai持续略有增加。结论抗性和易感植物之间的初始反应是非常不同的。“植物激素信号转导”途径对于Cf-19介导的免疫的形成很重要。此外,JA和SA均在调节Cf-19依赖性耐药反应中发挥作用。茉莉酸(JA)含量在第7天增加到非常高的水平,但随后在CGN18423中降低到第20天的初始水平,而在整个过程中从MM的0到20 dai持续略有增加。结论抗性和易感植物之间的初始反应是非常不同的。“植物激素信号转导”途径对于Cf-19介导的免疫的形成很重要。此外,JA和SA均在调节Cf-19依赖性耐药反应中发挥作用。茉莉酸(JA)含量在第7天增加到非常高的水平,但随后在CGN18423中降低到第20天的初始水平,而在整个过程中从MM的0到20 dai持续略有增加。结论抗性和易感植物之间的初始反应是非常不同的。“植物激素信号转导”途径对于Cf-19介导的免疫的形成很重要。此外,JA和SA均在调节Cf-19依赖性耐药反应中发挥作用。通路对于Cf-19介导的免疫的形成很重要。此外,JA和SA均在调节Cf-19依赖性耐药反应中发挥作用。通路对于Cf-19介导的免疫的形成很重要。此外,JA和SA均在调节Cf-19依赖性耐药反应中发挥作用。
更新日期:2019-12-20
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