Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing.

中文翻译：

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从人体组织切片中快速彻底地去除石蜡可提供增强的拉曼光谱和组织病理学分析。

从用于诊断组织学处理的组织中不完全去除石蜡和有机污染物一直是将拉曼光谱技术引入临床实践的严重障碍。我们报告了一种使用超级镜面不锈钢载玻片从一系列福尔马林固定的石蜡包埋组织中快速和完全去除石蜡的途径。该方法对一系列人类和动物组织同样有效，对存档和新样本同样有效，并且与标准病理学实验室程序兼容。我们描述了拉曼散射的一般增强和用免疫组织化学中用于临床诊断的抗体增强染色。