Design and synthesis of water-soluble near-infrared (NIR) emissive fluorescent molecules with aggregation-induced emission (AIE) characteristics, perfect signal-to-noise ratio for imaging of organelle, and photodynamic therapy (PDT) functions has received enormous attention. However, the dual-functional NIR AIEgens of high-fidelity tracking lysosome and ablation cancer cells was rarely reported. Herein, a series of AIE luminogens (AIEgens) with a typical AIE effect, good biocompatibility, superior photostability, high brightness, and excellent reactive oxygen species (ROS) generation ability were developed, which had different electronic push-pull strength and conjugate system size in the molecular structure. These AIEgens could specifically "light up" and dynamically long-term track the lysosomes in living cells and zebrafish with ultrahigh colocalization imaging Pearson's correlation coefficients (Rr: 0.9687) and overlap coefficient (R: 0.9967). Additionally, the MPAT of NIR luminescence as a photosensitizer was used for photodynamic ablation of cancer cells, owing to prompt generation of the ROS under green light irradiation (495-530 nm, 10 mW cm-2). Hence, this research not only expands the application range of NIR AIEgens but also provides useful insights into design of split-new method for the treatment of cancer.

中文翻译：

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用于细胞中溶酶体的高保真成像和光动力疗法的双功能NIR AIEgens。

具有聚集诱导发射（AIE）特性，用于细胞器成像的完美信噪比以及光动力疗法（PDT）功能的水溶性近红外（NIR）发射荧光分子的设计和合成受到了广泛的关注。然而，很少有人报道了高保真追踪溶酶体和消融癌细胞的双重功能的NIR AIEgens。本文开发了一系列具有典型AIE效应，良好的生物相容性，优异的光稳定性，高亮度和优异的活性氧（ROS）生成能力的AIE发光剂（AIEgens），它们具有不同的电子推挽强度和共轭体系尺寸在分子结构上。这些AIEgens可以专门“点亮” 并利用超高共定位成像Pearson的相关系数（Rr：0.9687）和重叠系数（R：0.9967）动态地长期跟踪活细胞和斑马鱼中的溶酶体。另外，由于在绿光照射下（495-530nm，10mWcm-2）迅速产生ROS，因此将NIR发光的MPAT用作光敏剂用于癌细胞的光动力消融。因此，这项研究不仅扩大了NIR AIEgens的应用范围，而且还为设计新的癌症治疗方法提供了有用的见识。由于在绿光照射下（495-530 nm，10 mW cm-2）迅速产生了ROS。因此，这项研究不仅扩大了NIR AIEgens的应用范围，而且还为设计新型的癌症治疗方法提供了有用的见识。由于在绿光照射下（495-530 nm，10 mW cm-2）迅速产生了ROS。因此，这项研究不仅扩大了NIR AIEgens的应用范围，而且还为设计新的癌症治疗方法提供了有用的见识。