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Plasma Membrane Domain Patterning and Self-Reinforcing Polarity in Arabidopsis.
Developmental Cell ( IF 11.8 ) Pub Date : 2019-12-13 , DOI: 10.1016/j.devcel.2019.11.015
Petra Marhava 1 , Ana Cecilia Aliaga Fandino 1 , Samuel W H Koh 1 , Adriana Jelínková 2 , Martina Kolb 3 , Dorina P Janacek 3 , Alice S Breda 1 , Pietro Cattaneo 1 , Ulrich Z Hammes 3 , Jan Petrášek 2 , Christian S Hardtke 1
Affiliation  

Cell polarity is a key feature in the development of multicellular organisms. For instance, asymmetrically localized plasma-membrane-integral PIN-FORMED (PIN) proteins direct transcellular fluxes of the phytohormone auxin that govern plant development. Fine-tuned auxin flux is important for root protophloem sieve element differentiation and requires the interacting plasma-membrane-associated BREVIS RADIX (BRX) and PROTEIN KINASE ASSOCIATED WITH BRX (PAX) proteins. We observed "donut-like" polar PIN localization in developing sieve elements that depends on complementary, "muffin-like" polar localization of BRX and PAX. Plasma membrane association and polarity of PAX, and indirectly BRX, largely depends on phosphatidylinositol-4,5-bisphosphate. Consistently, mutants in phosphatidylinositol-4-phosphate 5-kinases (PIP5Ks) display protophloem differentiation defects similar to brx mutants. The same PIP5Ks are in complex with BRX and display "muffin-like" polar localization. Our data suggest that the BRX-PAX module recruits PIP5Ks to reinforce PAX polarity and thereby the polarity of all three proteins, which is required to maintain a local PIN minimum.

中文翻译:

拟南芥中的等离子膜结构域图案和自增强极性。

细胞极性是多细胞生物发展中的关键特征。例如,非对称定位的血浆膜整合PIN形成(PIN)蛋白指导控制植物发育的植物激素生长素的跨细胞通量。微调的植物生长素通量对于根原卟啉筛分元素的分化很重要,并且需要相互作用的血浆膜相关BREVIS RADIX(BRX)和与BRX(PAX)蛋白相关的蛋白激酶。我们观察到筛子中“甜甜圈状”极性PIN的定位取决于BRX和PAX的互补,“松饼状”极性定位。PAX和间接BRX的质膜缔合和极性在很大程度上取决于磷脂酰肌醇-4,5-双磷酸酯。始终如一 磷脂酰肌醇-4-磷酸5激酶(PIP5Ks)中的突变体显示出与brx突变体相似的原位生物分化缺陷。相同的PIP5K与BRX配合使用,并显示“松饼状”极性定位。我们的数据表明,BRX-PAX模块募集PIP5K来增强PAX极性,从而增强所有三种蛋白质的极性,这是维持局部PIN最小值所需的。
更新日期:2019-12-19
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