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Mathematical modelingof ethanol production from glycerol by Enterobacter aerogenes concerning the influence of impurities, substrate, and product concentration
Biochemical Engineering Journal ( IF 3.9 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.bej.2019.107471
Juli Novianto Sunarno , Poonsuk Prasertsan , Wiriya Duangsuwan , Prawit Kongjan , Benjamas Cheirsilp

Abstract The application of biodiesel derived crude glycerol for ethanol production using Enterobacter aerogenes TISTR 1468 involved multiple interactions among substrate (glycerol), impurities (methanol) and product (ethanol and acetic acid). A mathematical model based on Monod and Luedeking-Piret models were established to simulate the cell performance in the presence of inhibitors. The Monod model was modified by adding the inhibition factors in terms of critical concentration (C*Ac nMeOH>nAc>nEtOH with the values of 119.06, 13.33, 1 and 0.0383, respectively). The Luedeking-Piret model confirmed that ethanol and acetic acid were predominantly growth-associated products. Glycerol up to 24.31 g/L and 31.32 g/L were optimum for cell growth and ethanol productivity, respectively. The cell performance was affected by methanol even at its lowest concentration tested (7.92 g/L). The applicability of the new two-phase growth model during the growth phase and stationary phase in the crude glycerol medium was validated and proved accurate (r2> 0.98).

中文翻译:

关于杂质、底物和产物浓度影响的产气肠杆菌从甘油生产乙醇的数学模型

摘要 生物柴油衍生的粗甘油在产气肠杆菌 TISTR 1468 乙醇生产中的应用涉及底物(甘油)、杂质(甲醇)和产物(乙醇和乙酸)之间的多重相互作用。建立了基于Monod和Luedeking-Piret模型的数学模型来模拟抑制剂存在下的细胞性能。Monod 模型通过添加临界浓度方面的抑制因子来修改(C*Ac nMeOH>nAc>nEtOH,其值分别为 119.06、13.33、1 和 0.0383)。Luedeking-Piret 模型证实乙醇和乙酸主要是与生长相关的产物。高达 24.31 g/L 和 31.32 g/L 的甘油分别是细胞生长和乙醇生产力的最佳选择。即使在测试的最低浓度 (7.92 g/L) 下,电池性能也会受到甲醇的影响。新的两阶段生长模型在粗甘油培养基中的生长期和稳定期的适用性得到验证并证明是准确的(r2> 0.98)。
更新日期:2020-03-01
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