BACKGROUND In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities. AIMS To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies. METHODS Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated. RESULTS Integral changes were generally small, but serum citrate signals decreased significantly in some samples. CONCLUSIONS Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

中文翻译：

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重新采集光谱后，血样中柠檬酸NMR峰的不可再现性。

背景技术在我们的代谢组学研究中，我们注意到在同一样品上重复进行NMR采集可导致代谢物信号强度改变。目的从两个大型人体代谢组学研究中，对血清和血浆中选定代谢物进行重复NMR采集的可重复性进行研究。方法对每种代谢物的两个峰区域进行积分，并对重新获得后发生的变化进行关联。结果总体变化很小，但某些样品中的血清柠檬酸信号明显降低。结论在某些重复光谱中，某些代谢产物积分不可重现。遵循已建立的协议，随机化分析顺序和生物标记物验证非常重要。