Suhuang antitussive capsule (SH), one of traditional Chinese patent medicines, has been widely used for treating cough variant asthma and postinfectious cough in clinic. The objective of this work is to identify the characteristic and active ingredients as the quality control markers for SH based on high performance liquid chromatography with photodiode array detector (HPLC-PDA) fingerprint and screening of anti-inflammatory components. Similarity analysis (SA), hierarchical clustering analysis (HCA) and principal component analysis (PCA) were used to evaluate 16 different batches of SH. 13 compounds accounting for 36% of the total components in the fingerprint were identified and semi-quantitatively analyzed, which anti-inflammatory activity was tested with the in vitro assay. The results showed that the established chemical fingerprint could clearly distinguish different batches of SH by SA, HCA, and PCA analysis. Furthermore, four known compounds (chlorogenic acid, schisandrin, angeloylgomisin H and praeruptorin A) were screened out to be the most discriminant variables, which could be applied to quality control of SH by quantitative analysis. The semi-quantitative results showed that six compounds were major components, i.e. arctiin (10.28 ± 3.18 mg/g), ephedrine (9.26 ± 1.58 mg/g), schisandrin (3.09 ± 0.83 mg/g), pseudoephedrine (2.34 ± 1.04 mg/g), schisandrin B (1.48 ± 0.16 mg/g), and 1-caffeoylquinic acid (1.36 ± 0.42 mg/g). The anti-inflammatory results showed that SH extract, praeruptorin A, schisandrin, arctigenin and pseudoephedrine could significantly inhibit inflammatory mediator NO production in LPS-stimulated RAW264.7 macrophages. These findings indicated that praeruptorin A, schisandrin, arctiin and pseudoephedrine could be proposed as the quality control markers for SH.

中文翻译：

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基于HPLC-PDA指纹图谱和消炎筛选的苏黄镇咳胶囊质量控制标志物的鉴定。

苏皇镇咳胶囊（SH）是中成药之一，在临床上已广泛用于治疗咳嗽变异性哮喘和感染后咳嗽。这项工作的目的是基于具有光电二极管阵列检测器（HPLC-PDA）指纹的高效液相色谱法和抗炎成分的筛选，确定特征性和有效成分作为SH的质量控制标记。使用相似度分析（SA），层次聚类分析（HCA）和主成分分析（PCA）评估16种不同批次的SH。鉴定并半定量分析了13种化合物，占指纹中总成分的36％，并通过体外测定法测试了其抗炎活性。结果表明，所建立的化学指纹图谱可以通过SA，HCA和PCA分析清楚地区分不同批次的SH。此外，筛选出四种已知化合物（绿原酸，五味子苷，当归果糖球蛋白H和praeruptorin A）是最有区别的变量，可通过定量分析将其应用于SH的质量控制。半定量结果表明，六种化合物是主要成分，即牛黄素（10.28±3.18 mg / g），麻黄碱（9.26±1.58 mg / g），五味子素（3.09±0.83 mg / g），伪麻黄碱（2.34±1.04 mg / g），五味子素B（1.48±0.16 mg / g）和1-咖啡酰奎尼酸（1.36±0.42 mg / g）。抗炎结果表明，SH提取物，praeruptorin A，五味子素，牛ti皂苷元和伪麻黄碱可显着抑制LPS刺激的RAW264.7巨噬细胞中炎性介质NO的产生。这些发现表明，praeruptorin A，五味子素，arctiin和伪麻黄碱可以作为SH的质量控制标志物。