The present study was designed to investigate the effect of topical erythropoietin on the healing process of induced necrotizing scleritis and to evaluate the ocular side effects of this treatment modality in a rabbit model. Necrotizing scleritis was induced in 8 New Zealand albino rabbits. The animals were then randomly divided into one of two groups: a treated group administered a topical erythropoietin-containing cellulose-based gel every 8 h or a control group treated with a cellulose-based gel without erythropoietin every 8 h. The sizes of the lesions measured at different time points were compared between the groups. After three months, the rabbits' eyes were enucleated and histologically and immunohistochemically evaluated for angiogenesis and apoptosis. The lesions were completely vascularized in all eyes of the treated group and 50% of eyes of the control group. The mean interval from the induction of scleral necrosis to a complete improvement was 28 days in the treated group and 62.5 days in the control group (P = 0.04). Histological examination revealed that erythropoietin enhanced the improvement of necrotizing scleritis by stimulating angiogenesis and reducing apoptosis. Neovascularization of the cornea, iris, or retina was not observed in the treated group. We observed a significantly faster recovery to complete improvement of necrotizing scleritis in rabbit eyes treated with erythropoietin compared to those of the control group. Treated eyes had a higher rate of complete healing and had no ocular safety concerns. This therapeutic modality represents a promising treatment for scleral necrosis following various types of ocular surgery.

中文翻译：

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局部促红细胞生成素对家兔模型中实验性诱导的无血管巩膜损伤愈合的影响。

本研究旨在研究局部促红细胞生成素在诱导性坏死性巩膜炎愈合过程中的作用，并评估这种治疗方式在兔模型中的眼部副作用。在8只新西兰白化兔中诱发了坏死性巩膜炎。然后将动物随机分为两组之一：治疗组每8 h施用一次局部用含促红细胞生成素的纤维素基凝胶，或对照组，每8 h用不含促红细胞生成素的纤维素基凝胶治疗。两组之间在不同时间点测量的病变大小进行了比较。三个月后，摘除兔子的眼睛，并在组织学和免疫组织化学方面评估兔子的血管生成和凋亡。在治疗组的所有眼睛和对照组的50％的眼睛中，病变都被完全血管化。从诱导巩膜坏死到完全好转的平均间隔在治疗组为28天，在对照组为62.5天（P = 0.04）。组织学检查显示，促红细胞生成素通过刺激血管生成和减少细胞凋亡来增强坏死性巩膜炎的改善。在治疗组中未观察到角膜，虹膜或视网膜的新血管形成。我们观察到，与对照组相比，用促红细胞生成素治疗的兔眼恢复完全改善坏死性巩膜炎的速度明显更快。经过治疗的眼睛具有更高的完全治愈率，并且没有眼部安全隐患。