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Leptomycin B inhibits the proliferation, migration, and invasion of cultured gastric carcinoma cells.
Bioscience, Biotechnology, and Biochemistry ( IF 1.6 ) Pub Date : 2019-10-16 , DOI: 10.1080/09168451.2019.1673148
Hepan Zhu 1 , Yi Yang 2 , Li Wang 2 , Xiaobin Xu 2 , Tingting Wang 2 , Haoran Qian 1
Affiliation  

Chromosome region maintenance 1 (CRM1) plays a critical role in tumorigenesis and progression through modulating nuclear export of several proteins. However, the precise effects of CRM1 inhibitor on gastric carcinoma have not yet been illustrated. Here, we investigated the potential anti-cancer activities of leptomycin B, the most potent CRM1 antagonist, on cultured gastric carcinoma cells. Our findings demonstrate that CRM1 was highly expressed in four gastric carcinoma cell lines. Leptomycin B inhibited the viability of HGC-27 and AGS cells in a dose- and time-dependent pattern. Leptomycin B at the dose of 10 nM or 100 nM suppressed the migration and invasion of HGC-27 and AGS cells. Leptomycin B elevated the expressions of autophagy-related protein LC3-II and autophagy substrate p62. Moreover, leptomycin B enhanced the LC3-positive puncta formation in cells. Our data suggest that leptomycin B may exert an anti-cancer activity possibly through interfering autophagy function in gastric carcinoma cells.

中文翻译:

Leptomycin B抑制培养的胃癌细胞的增殖,迁移和侵袭。

染色体区域维持1(CRM1)通过调节几种蛋白质的核输出在肿瘤发生和发展中起关键作用。但是,尚未证明CRM1抑制剂对胃癌的确切作用。在这里,我们调查了最有效的CRM1拮抗剂瘦霉素B对培养的胃癌细胞的潜在抗癌活性。我们的发现表明,CRM1在四种胃癌细胞系中高表达。瘦霉素B以剂量和时间依赖性方式抑制HGC-27和AGS细胞的活力。浓度为10 nM或100 nM的细霉素B抑制了HGC-27和AGS细胞的迁移和侵袭。Leptomycin B升高自噬相关蛋白LC3-II和自噬底物p62的表达。而且,瘦霉素B增强了细胞中LC3阳性点的形成。我们的数据表明,瘦素B可能通过干扰胃癌细胞中的自噬功能发挥抗癌活性。
更新日期:2019-12-19
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