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Preparation and effects on neuronal nutrition of plasmenylethonoamine and plasmanylcholine from the mussel Mytilus edulis.
Bioscience, Biotechnology, and Biochemistry ( IF 1.6 ) Pub Date : 2019-10-12 , DOI: 10.1080/09168451.2019.1674632
Yi Ding 1 , Rui Wang 1 , Xiaoxu Wang 1 , Peixu Cong 1 , Yanjun Liu 1 , Zhaojie Li 1 , Jie Xu 1 , Changhu Xue 1, 2
Affiliation  

Plasmenylethonoamine (pPE) and plasmanylcholine (aPC) are important phospholipid subclasses. Herein we explored optimum conditions for enzymatic purification and preparation of pPE and aPC from the mussel Mytilus edulis and bovine brain. Among them, pPE in Mytilus edulis PE was mainly p18:0-20:5 and p18:0-22:6, and its purity was 92.7%; aPC in PC was primarily a16:0-22:6 and a16:0-20:5, and aPC accounted for 90.2% of PC. We thereafter evaluated neurotrophic effects of Mytilus edulis pPE, aPC, and bovine brain pPE in a NGF-induced PC12 cell model. Morphologically, pPE and aPC could both promote differentiation, manifested in a significant increase in neurite length and number, due to increased expression of synaptophysin and growth protein GAP-43 in a dose-independent and structure-selective manner. Importantly, the effect on neuronal nutrition of pPE was better than aPC, and marine pPE was better than terrestrial pPE, which might be ascribed to vinyl-ether bond and differences in fatty acid composition.Abbreviations: AA: arachidonic acid; DHA: docosahexaenoic acid; EIC: extracted ion chromatogram; EPA: eicosapentanoic acid; GAP: growth-associated protein; HPLC: high-performance liquid chromatography; LC-MS/MS: liquid chromatography-tandem mass spectrometry; LPC: lyso-PC; LPE: lyso-PE; MS: mass spectrometry; NGF: nerve growth factor; PC: phosphatidylcholine; aPC: plasmanylcholine; PE: phosphatidylethanolamine; pPE: plasmenylethonoamine; PG: phosphoglycerols; PLs: phospholipids; PS: phosphoserines; TIC: total ion chromatogram.

中文翻译:

贻贝贻贝的血浆烯丙基乙胺和血浆甲胆碱的制备及其对神经元营养的影响。

血浆视紫红胺(pPE)和血浆甲胆碱(aPC)是重要的磷脂亚类。本文中,我们探索了从贻贝贻贝和牛脑中酶法纯化和制备pPE和aPC的最佳条件。其中,紫花苜蓿PE中的pPE主要为p18:0-20:5和p18:0-22:6,纯度为92.7%。PC中的aPC主要是a16:0-22:6和a16:0-20:5,aPC占PC的90.2%。此后,我们在NGF诱导的PC12细胞模型中评估了食用菌Mytilus edulis pPE,aPC和牛脑pPE的神经营养作用。从形态上讲,由于突触素和生长蛋白GAP-43的表达以剂量独立和结构选择性的方式增加,pPE和aPC均可促进分化,表现为神经突长度和数量的显着增加。重要的,pPE对神经营养的影响优于aPC,海洋pPE优于陆生pPE,这可能归因于乙烯基醚键和脂肪酸组成的差异。DHA:二十二碳六烯酸;EIC:提取离子色谱图;EPA:二十碳五烯酸;GAP:与生长相关的蛋白质;HPLC:高效液相色谱法;LC-MS / MS:液相色谱-串联质谱; LC-MS / MS。LPC:溶血-PC;LPE:溶血-PE;MS:质谱。NGF:神经生长因子;PC:磷脂酰胆碱;aPC:血浆胆碱;PE:磷脂酰乙醇胺;pPE:枯烯基乙胺;PG:磷酸甘油;PL:磷脂;PS:磷酸丝氨酸;TIC:总离子色谱图。缩写:AA:花生四烯酸;其可以归因于乙烯基-醚键和脂肪酸组成的差异。DHA:二十二碳六烯酸;EIC:提取离子色谱图;EPA:二十碳五烯酸;GAP:与生长相关的蛋白质;HPLC:高效液相色谱法;LC-MS / MS:液相色谱-串联质谱; LC-MS / MS。LPC:溶血-PC;LPE:溶血-PE;MS:质谱。NGF:神经生长因子;PC:磷脂酰胆碱;aPC:血浆胆碱;PE:磷脂酰乙醇胺;pPE:枯烯基乙胺;PG:磷酸甘油;PL:磷脂;PS:磷酸丝氨酸;TIC:总离子色谱图。缩写:AA:花生四烯酸;其可以归因于乙烯基-醚键和脂肪酸组成的差异。DHA:二十二碳六烯酸;EIC:提取离子色谱图;EPA:二十碳五烯酸;GAP:与生长相关的蛋白质;HPLC:高效液相色谱法;LC-MS / MS:液相色谱-串联质谱; LC-MS / MS。LPC:溶血-PC;LPE:溶血-PE;MS:质谱。NGF:神经生长因子;PC:磷脂酰胆碱;aPC:血浆胆碱;PE:磷脂酰乙醇胺;pPE:枯烯基乙胺;PG:磷酸甘油;PL:磷脂;PS:磷酸丝氨酸;TIC:总离子色谱图。液相色谱-串联质谱;LPC:溶血-PC;LPE:溶血-PE;MS:质谱。NGF:神经生长因子;PC:磷脂酰胆碱;aPC:血浆胆碱;PE:磷脂酰乙醇胺;pPE:枯烯基乙胺;PG:磷酸甘油;PL:磷脂;PS:磷酸丝氨酸;TIC:总离子色谱图。液相色谱-串联质谱;LPC:溶血-PC;LPE:溶血-PE;MS:质谱。NGF:神经生长因子;PC:磷脂酰胆碱;aPC:血浆胆碱;PE:磷脂酰乙醇胺;pPE:枯烯基乙胺;PG:磷酸甘油;PL:磷脂;PS:磷酸丝氨酸;TIC:总离子色谱图。
更新日期:2019-12-19
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