This study was conducted to evaluate the protective effects of chamazulene against IL-1β-induced rat primary chondrocytes and complete Freund's adjuvant (CFA)-induced osteoarthritic inflammation in rats. Oxidative stress markers, pro-inflammatory cytokines, and regulatory proteins were measured. Chamazulene significantly reverted (p < 0.05) the levels of lipid peroxidation and enhanced the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) enzymes against IL-1β and CFA-induced oxidative stress. The levels of TNF-α and IL-6 were reduced (p < 0.05) in chamazulene treatment against IL-1β and CFA-induced inflammation. Western blot analysis results on the expressions of MMP-3, MMP-9, p65 NF-kβ, iNOS, and COX-2 showed chamazulene was able to protect the chondrocytes against IL-1β-induced osteoarthritic inflammation. Histopathology of rat hind ankle showed chamazulene significantly protected against CFA-induced osteoarthritic inflammation. Therefore, chamazulene can be recommended as a therapeutic agent for clinical trials against osteoarthritic inflammation.

中文翻译：

---

在体外和体内模型中，Chamazulene通过调节基质金属蛋白酶（MMP）和NF-kβ途径逆转骨关节炎炎症。

进行了这项研究，以评估甲磺灵对IL-1β诱导的大鼠原代软骨细胞和完全弗氏佐剂（CFA）诱导的大鼠骨关节炎的保护作用。测量了氧化应激标志物，促炎细胞因子和调节蛋白。Chamazulene显着还原（p <0.05）脂质过氧化水平，并增强了针对IL-1β和CFA诱导的氧化应激的超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）酶的活性。在Chamazulene对抗IL-1β和CFA诱导的炎症的治疗中，TNF-α和IL-6的水平降低（p <0.05）。蛋白质印迹分析结果显示MMP-3，MMP-9，p65NF-kβ，iNOS，COX-2的结果表明chamazulene能够保护软骨细胞免受IL-1β诱导的骨关节炎的炎症。大鼠后踝的组织病理学研究显示，马甲富烯对CFA诱导的骨关节炎炎症具有明显的保护作用。因此，可以推荐使用马甲磺胺作为抗骨关节炎炎症的临床试验的治疗剂。