To develop an iPSC SOX9 reporter line for monitoring differentiation into SOX9 expressing cells such as chondrocytes, cranial neural crest and Sertoli cells, we used gene editing to introduce sequences encoding the tdTomato fluorescent protein into the SOX9 locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. Endogenous SOX9 expression was undisturbed and the tdTomato fluorescent reporter mirrored SOX9 mRNA expression. This iPSC line will be useful for assessing iPSC differentiation into SOX9-expressing cells and enrichment by cell sorting.

为了开发iPSC SOX9报告基因系以监测向表达SOX9的细胞（如软骨细胞，颅神经rest和Sertoli细胞）的分化，我们使用基因编辑将编码tdTomato荧光蛋白的序列引入SOX9基因座。经基因编辑的品系具有正常的核型，表达多能性标记并分化为代表三个胚芽层的细胞。内源性SOX9表达不受干扰，tdTomato荧光报告基因反映了SOX9 mRNA表达。该iPSC品系可用于评估iPSC分化为表达SOX9的细胞以及通过细胞分选进行富集。