The development of new ion-activation/dissociation methods continues to be one of the most active areas of mass spectrometry owing to the broad applications of tandem mass spectrometry in the identification and structural characterization of molecules. This Review will showcase the impact of ultraviolet photodissociation (UVPD) as a frontier strategy for generating informative fragmentation patterns of ions, especially for biological molecules whose complicated structures, subtle modifications, and large sizes often impede molecular characterization. UVPD energizes ions via absorption of high-energy photons, which allows access to new dissociation pathways relative to more conventional ion-activation methods. Applications of UVPD for the analysis of peptides, proteins, lipids, and other classes of biologically relevant molecules are emphasized in this Review.

中文翻译：

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紫外光解离质谱分析生物分子。

由于串联质谱在分子的鉴定和结构表征中的广泛应用，新的离子活化/解离方法的发展仍然是质谱最活跃的领域之一。这篇综述将展示紫外线光解离（UVPD）的影响，作为产生离子的信息性碎裂模式的前沿策略，尤其是对于结构复杂，修饰细微且尺寸大通常会阻碍分子表征的生物分子。UVPD通过吸收高能光子来激发离子，相对于更常规的离子激活方法，这可以进入新的解离途径。UVPD在分析肽，蛋白质，脂质，