BACKGROUND Despite the wide use of dental materials for CAD/CAM system in prosthetic treatment, the effect of the materials, which are used as dental implants core fabricated, on cells involved in dental implant osseointegration is uncertain. This study aimed to investigate and compare the effect of single core materials used for dental implants fabricated by the dental prostheses fabrication process and the CAD/CAM milling method on MC3T3-E1 cells. METHODS The materials used for prostheses restoration in this experiment were Porcelain Fused Gold (P.F.G), Lithium disilicate glass ceramic (LiSi2), Zirconia (ZrO2), Nickel-Chromium (Ni-Cr) and Cobalt-Chromium (Co-Cr). MC3T3-E1 cells were cultured and used, the cell adhesion and morphology were observed and analyzed using confocal laser scanning microscopy (CLSM). Methoxyphenyl tetrazolium salt (MTS) and alkaline phosphatase (ALP) assay were used to observe the cell proliferation and differentiation. RESULTS CLSM revealed irregular cell adhesion and morphology and the filopodia did not spread in the Ni-Cr specimen group. Significantly high cell proliferation was observed in the ZrO2 specimen group. The LiSi2 specimen group presented significantly high cell differentiation. Intergroup comparison of cell proliferation and differentiation between the Ni-Cr specimen group and all other specimen groups showed significant differences (p < .05). CONCLUSION Cell proliferation and differentiation were observed from the cores, which were fabricated with all specimen groups on cytocompatibility except the Ni-Cr specimen group.

中文翻译：

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牙科植入物的核心制造核心材料对MC3T3-E1细胞体外细胞相容性的影响。

背景技术尽管在义齿治疗中广泛使用了用于CAD / CAM系统的牙科材料，但是用作牙科植入物核心的材料对涉及牙科植入物骨整合的细胞的影响尚不确定。这项研究旨在调查和比较通过义齿制造工艺和CAD / CAM铣削方法制造的用于牙科植入物的单芯材料对MC3T3-E1细胞的影响。方法本实验中用于修复假体的材料为瓷熔金（PFG），二硅酸锂玻璃陶瓷（LiSi2），氧化锆（ZrO2），镍铬（Ni-Cr）和钴铬（Co-Cr）。培养并使用MC3T3-E1细胞，使用共聚焦激光扫描显微镜（CLSM）观察和分析细胞粘附和形态。甲氧苯基四唑鎓盐（MTS）和碱性磷酸酶（ALP）测定法观察细胞的增殖和分化。结果CLSM显示Ni-Cr标本组细胞黏附和形态不规则，丝状伪足未扩散。在ZrO2标本组中观察到明显的高细胞增殖。LiSi2标本组显示出明显的高细胞分化。Ni-Cr标本组与所有其他标本组之间的细胞增殖和分化的组间比较显示出显着差异（p <.05）。结论观察到细胞增殖和分化的核心，除了镍铬样品组外，所有样品组均具有细胞相容性。