The present study was designed to evaluate the radioprotective effect of diethylcarbamazine (DEC) against oxidative stress and acute lung injury induced by total body radiation (TBI) in mice. For study the optimum dose for radiation protection of DEC, mice were administrated with three dose of DEC (10, 50 and 100 mg/kg), once daily for eight consecutive days. Animals were exposed whole body to 5 Gy X-radiation on the 9 day. The radioprotective potential of DEC in lung tissues was assessed using oxidative stress examinations at 24 h after TBI and histopathological assay also was analyzed one week after TBI. Results from biochemical analyses demonstrated increased malonyldialdehyde (MDA), nitric oxide (NO) and protein carbonyl (PC) levels of lung tissues in only irradiated group. Histopathologic findings also showed an increase in the number of inflammatory cells and the acute lung injury in this group. DEC pretreatment significantly mitigated the oxidative stress biomarkers as well as histological damages in irradiated mice. The favorable radioprotective effect against lungs injury was observed at a dose of 10 mg/kg of DEC in mice as compared with two other doses (50 and 100 mg/kg). The data of this study showed that DEC at a dose of 10 mg/kg with having antioxidant and anti-inflammatory properties can be used as a therapeutic candidate for protecting the lung from radiation-induced damage.

中文翻译：

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二乙基卡巴嗪对小鼠辐射诱发的急性肺损伤和氧化应激的辐射防护作用。

本研究旨在评估二乙基卡巴嗪（DEC）对小鼠全身辐射（TBI）引起的氧化应激和急性肺损伤的辐射防护作用。为了研究用于DEC辐射防护的最佳剂量，连续三天每天一次给小鼠服用三剂DEC（10、50和100 mg / kg）。在第9天，使动物全身暴露于5 Gy X射线。在TBI后24小时使用氧化应激检查评估DEC在肺组织中的放射防护能力，并在TBI后一周进行组织病理学分析。生化分析结果表明，仅辐照组的肺组织丙二醛（MDA），一氧化氮（NO）和蛋白质羰基（PC）含量增加。组织病理学结果还显示该组炎症细胞数量增加和急性肺损伤。DEC预处理显着减轻了辐照小鼠的氧化应激生物标志物以及组织学损害。与其他两种剂量（50和100 mg / kg）相比，在小鼠中DEC的剂量为10 mg / kg时，观察到了对肺损伤的有利放射防护作用。这项研究的数据表明，具有抗氧化和抗炎特性的DEC剂量为10 mg / kg可用作保护肺部免受辐射引起的损害的治疗候选物。与其他两种剂量（50和100 mg / kg）相比，在小鼠中DEC的剂量为10 mg / kg时，观察到了对肺损伤的有利放射防护作用。这项研究的数据表明，具有抗氧化和抗炎特性的DEC剂量为10 mg / kg可用作保护肺部免受辐射引起的损害的治疗候选物。与其他两种剂量（50和100 mg / kg）相比，在小鼠中DEC的剂量为10 mg / kg时，观察到了对肺损伤的有利放射防护作用。这项研究的数据表明，具有抗氧化和抗炎特性的DEC剂量为10 mg / kg可用作保护肺部免受辐射引起的损害的治疗候选物。