This study examined diamondback moth (Plutella xylostella) strains showing high-level resistance to cyantraniliprole (KA17 strain) and to flubendiamide and chlorantraniliprole (KU13 strain). The LC50 value of the KA17 strain against cyantraniliprole was ca. 100-fold higher than that of the KU13 strain. The KA17 strain also exhibited high-level resistance to chlorantraniliprole and flubendiamide equivalent to those in the KU13 strain. The KU13 strain showed a higher LC50 value against cyantraniliprole than the susceptible strains. However, the LC50 value of the KU13 strain against cyantraniliprole was below the agriculturally recommended concentration. Subsequent QTL analysis using ddRAD-seq identified the resistance responsible regions of the KA17 and KU13 strains with different diamide resistance profiles. Ryanodine receptor (RyR) gene was included in the identified regions. Single nucleotide polymorphism calling in the RyR gene using RNA-seq found previously reported G4946E (amino acid mutation from glycine to glutamic acid at amino acid position 4946) and novel I4790K (amino acid mutation from isoleucine to lysine at amino acid position 4790) mutations, respectively, in the RyR of the KU13 and KA17 strains. Functional significance of I4790K in the resistance was confirmed in calcium imaging of the human embryonic kidney 293T cell line expressing Bombyx mori RyR with the mutation. This reporting is the first describing I4790K as a fundamental mechanism responsible for the resistance to the diamides including cyantraniliprole. From this study, we also report up-regulated expression of some degradation enzymes and that of the RyR gene in the KA17 and KU13 strains based on results of RNA-seq data analysis.

中文翻译：

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Ryanodine受体突变（G4946E和I4790K）在小菜蛾小菜蛾Plutella xylostella L.中对二酰胺杀虫剂抗性的不同作用。

这项研究检查了小菜蛾（Plutella xylostella）菌株，它们对氰基氰基丙烯腈（KA17菌株）以及对氟苯二酰胺和氯氰基丙烯腈（KU13菌株）具有高水平的抗性。KA17菌株对氰基traniliprole的LC50值为ca。比KU13菌株高100倍。KA17菌株还显示出与KU13菌株相同的高水平对氯氰基丙烯腈和氟苯二酰胺的抗性。KU13菌株对氰基氰基丙烯腈的LC50值比易感菌株更高。但是，KU13菌株对氰基氰基丙烯腈的LC50值低于农业推荐的浓度。随后使用ddRAD-seq进行的QTL分析确定了具有不同二酰胺抗性谱的KA17和KU13菌株的抗性负责区域。在确定的区域中包括了Ryanodine受体（RyR）基因。使用RNA-seq调用RyR基因的单核苷酸多态性先前发现了G4946E（氨基酸位置4946的甘氨酸到谷氨酸的氨基酸突变）和新型I4790K（氨基酸位置4790的异亮氨酸到赖氨酸的氨基酸突变）突变，分别在KU13和KA17菌株的RyR中。在表达具有该突变的家蚕RyR的人胚胎肾293T细胞系的钙成像中，证实了I4790K在抗性中的功能重要性。该报道首次将I4790K描述为对包括氰基氰基丙烯腈在内的二酰胺具有抗性的基本机制。根据这项研究，