OBJECTIVE This study aims to evaluate sequence-modified Ti surfaces functionalized with silanized glutaraldehyde and further grafted with the active biomolecules of phosphatidylcholine and type I collagen (COL I). METHODS The properties of the functional surfaces were investigated by various surface analysis techniques and characterized their capability in osteogenic cell attachment, differentiation, and mineralization in vitro. RESULTS The Ti surfaces grafted with phosphatidylcholine and COL I effectively improved the hydrophilicity. In addition, an effect of COL I concentrations (higher than 2.5μg/mL) do not stimulate subsequent alkaline phosphatase (ALP) activity during osteogenesis in vitro. However, the result is different in phosphatidylcholine, that is, as the concentration of phosphatidylcholine increased enhances subsequent osteogenetic properties. The Ti groups with bioactive molecules affected cell characteristics in vitro in contrast to the controlled Ti group. The proliferation and differentiation levels of osteoprogenetor cells were enhanced and ALP was strongly expressed in the groups grafted with phosphatidylcholine and COL I. SIGNIFICANCE This modification promotes progenitor bone cell adhesion, proliferation, and differentiation and thus drastically improves the success rate for implant modification by accelerating surface osseointegration.

中文翻译：

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硅烷化戊二醛官能化钛表面与磷脂酰胆碱和I型胶原移植物的体外评估和比较效果。

目的本研究旨在评估经硅烷化的戊二醛功能化并进一步嫁接有磷脂酰胆碱和I型胶原（COL I）的活性生物分子的序列修饰的Ti表面。方法通过各种表面分析技术研究功能性表面的特性，并表征其在体外对成骨细胞附着，分化和矿化的能力。结果钛表面接枝了磷脂酰胆碱和COL I有效地改善了亲水性。此外，在体外成骨过程中，COL I浓度（高于2.5μg/ mL）的作用不会刺激随后的碱性磷酸酶（ALP）活性。但是，磷脂酰胆碱的结果是不同的，即，因为磷脂酰胆碱的浓度增加会增强随后的成骨特性。与对照组相比，具有生物活性分子的Ti基团在体外影响细胞特性。在移植了磷脂酰胆碱和COL I的组中，骨祖细胞的增殖和分化水平得到增强，ALP得以强烈表达。表面骨整合。