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Sinorhizobium fredii HH103 syrM inactivation affects the expression of a large number of genes, impairs nodulation with soybean and extends the host-range to Lotus japonicus.
Environmental Microbiology ( IF 5.1 ) Pub Date : 2019-12-17 , DOI: 10.1111/1462-2920.14897
Sebastián Acosta-Jurado 1 , Cynthia Alias-Villegas 1 , Pilar Navarro-Gómez 1 , Andrés Almozara 1 , Miguel A Rodríguez-Carvajal 2 , Carlos Medina 1 , José-María Vinardell 1
Affiliation  

Sinorhizobium fredii HH103 RifR is a broad host-range rhizobial strain able to nodulate with soybean and Lotus burttii, but it is ineffective with L. japonicus. Here, we study the role of the HH103 RifR SyrM protein in the regulation of gene expression and its relevance in symbiosis with those three legumes. RNAseq analyses show that HH103 SyrM is an important transcriptional regulator not only in the presence of inducer flavonoids but also in its absence. Lack of SyrM increases Nod factors production and decreases genistein-mediated repression of exopolysaccharide production in HH103. In symbiosis, mutation of syrM partially impaired interaction with soybean but improves effectiveness with L. burttii and extends the host-rage to L. japonicus Gifu. In addition, HH103 syrM mutants enter in both Lotus species by infection threads, whereas HH103 uses the more primitive intercellular infection to enter into L. burttii roots These symbiotic phenotypes were previously observed in two other HH103 mutants affected in symbiotic regulators, nodD2 and nolR, revealing that in S. fredii HH103 numerous transcriptional regulators finely modulate symbiotic gene expression.

中文翻译:

费氏中华根瘤菌HH103 syrM失活会影响大量基因的表达,损害大豆的结瘤作用,并将宿主范围扩展至日本莲。

fredii中华根瘤菌HH103 RifR是一种广泛的寄主范围的根瘤菌菌株,能够与大豆和莲Lotus结节,但对日本乳杆菌无效。在这里,我们研究了HH103 RifR SyrM蛋白在调节基因表达中的作用及其与这三种豆类共生的相关性。RNAseq分析显示,HH103 SyrM不仅在存在诱导剂类黄酮的情况下而且在其不存在时都是重要的转录调节因子。SyrM的缺乏增加了HH103中Nod因子的产生,并降低了染料木素介导的外多糖产生的抑制。在共生中,syrM的突变部分削弱了与大豆的相互作用,但提高了与伯氏乳杆菌的效力,并将宿主范围扩展到日本岐阜。此外,HH103 syrM突变体通过感染途径进入两种莲花物种,
更新日期:2020-03-02
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