This study was aimed to determine the effects of boric acid on oxidative stress, testicular tissue and spermatozoon DNA. Experiments were performed with Swiss Albino mice divided equally into two groups based on the tratment period: one for 4 and the other for 6-week duration. These groups were further divided into subgroups as Control and those administered daily at oral doses of 115 mg/kg, 250 mg/kg and 450 mg/kg of boric acid. Then, testicular tissue were examined postmortem and analyzed using ex-vivo biochemical tools for oxidative stress, spermatozoon membrane integrity, sperm motility and live cell rate (%). In both 4 and 6-week groups, v. seminalis weight, membrane integrity, motility, live cells and GSH levels exhibited a decreasing trent compared to the controls. In addition, 6-week group had a decrease in SOD level. MDA level was higher in controls in both 4 and 6-week groups. Spermatozoon DNA was intact in the 4-week group, but damaged in the 6-week group, and the degree of the damage dependent on the administered dose. Boric acid induces oxidative stress in testicular tissue, and its long-term application (only 6 weeks) caused damage in spermatozoon DNA.

中文翻译：

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硼酸喂养对小鼠睾丸氧化应激参数，精子参数和DNA损伤的影响。

这项研究旨在确定硼酸对氧化应激，睾丸组织和精子DNA的影响。实验根据瑞士白化病小鼠的治疗时间分为两组：一组为4只，另一组为6周。这些组进一步分为亚组作为对照组，每天以115 mg / kg，250 mg / kg和450 mg / kg硼酸的口服剂量给药。然后，在死后检查睾丸组织，并使用离体生化工具分析其氧化应激，精子膜完整性，精子活力和活细胞率（％）。在4周和6周组中，与对照组相比，精浆重量，膜完整性，运动性，活细胞和谷胱甘肽水平显示出减少的tr。另外，第6周组的SOD水平降低了。在4周和6周组中，对照组的MDA水平均较高。精子DNA在4周组中是完整的，但在6周组中是受损的，并且损伤的程度取决于给药剂量。硼酸在睾丸组织中引起氧化应激，长期使用（仅6周）会导致精子DNA受损。