Homogenous nanostructuration of molecularly imprinted polymer (MIP) films for follicle-stimulating hormone (FSH)-sensing was achieved by using optimized colloidal crystals as a hard mold. Introduction of a heating step after assembling colloidal crystals of silica beads promoted their adhesion. Thus, precise assembling of beads was not disturbed during further multisteps of surface imprinting, and crack-free hexagonal packing was maintained. Scanning electron microscopy imaging confirmed hexagonal packing of silica colloidal crystals as well as homogenous nanostructuration in MIP films. FSH immobilization over silica beads and later its derivatization with electroactive functional monomers was confirmed by X-ray photoelectron spectroscopy analysis. The nanostructured molecular recognition films prepared in this way were combined with an electrochemical transducer in order to design a capacitive impedimetry-based chemosensing system. It was tested for the determination of FSH in the range from 0.1 fM to 100 pM in 10 mM 2-(N-morpholino) ethane sulfonic acid buffer (pH = 4.2). The detection limit of the chemosensor was 0.1 fM, showing a high selectivity with respect to common protein interferences as well as other protein hormones of the gonadotropin family.

中文翻译：

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六边形堆积的大孔分子印迹聚合物，用于刺激卵泡的激素蛋白的化学传感。

通过使用优化的胶体晶体作为硬模，实现了分子印迹聚合物（MIP）膜的均相纳米结构，用于卵泡刺激激素（FSH）传感。组装硅胶珠的胶体晶体后引入加热步骤可增强其附着力。因此，在进一步的多步表面压印过程中，不影响小珠的精确组装，并保持了无裂纹的六角形堆积。扫描电子显微镜成像证实了二氧化硅胶体晶体的六方堆积以及MIP膜中的均匀纳米结构。通过X射线光电子能谱分析证实了FSH固定在二氧化硅微珠上，随后被电活性功能单体衍生化。将以此方式制备的纳米结构分子识别膜与电化学换能器组合，以设计基于电容阻抗法的化学传感系统。在10 mM 2-（N-吗啉代）乙烷磺酸缓冲液（pH = 4.2）中测试了从0.1 fM到100 pM范围内FSH的测定。化学传感器的检测极限为0.1 fM，相对于常见的蛋白质干扰素和促性腺激素家族的其他蛋白质激素，显示出很高的选择性。