Abstract Aqueous two-phase extraction (ATPE) has been used since the 1960s, but even having advantages as high recovery yields and selectivity and relatively low cost, it has not been extensively adopted by the biotechnological industry. Recently, the production of bioproducts is entering a new era, where the continuous mode processes are spreading, and the high degree of integration of the ATPE makes them suitable for this kind of processes, however, few studies were done. The following review presents the brief history and the key challenges of continuous ATPE until nowadays and the main devices used, discussing the importance of the microfluidics in the study and development of continuous ATPE, and it analyses the future challenges. In the end, it can be concluded that continuous ATPE has not overcome some major challenges, but it will be an important tool in the downstream processing of biomolecules.

中文翻译：

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连续水相两相萃取：从微流体到集成生物制造

摘要 水相两相萃取（ATPE）自1960年代开始使用，但即使具有回收率和选择性高、成本相对较低的优点，但并未被生物技术行业广泛采用。最近，生物制品的生产进入了一个新的时代，连续模式工艺正在普及，而ATPE的高度集成使其适用于这种工艺，但很少有人进行研究。以下综述介绍了连续 ATPE 的简要历史和迄今为止的主要挑战以及使用的主要设备，讨论了微流体在连续 ATPE 研究和发展中的重要性，并分析了未来的挑战。最后可以得出结论，持续的ATPE并没有克服一些重大挑战，