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Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration
Journal of Industrial and Engineering Chemistry ( IF 6.1 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.jiec.2019.12.005
Min Suk Lee , Dong Hyun Lee , Jin Jeon , Giyoong Tae , Young Min Shin , Hee Seok Yang

Abstract In order to promote effective healing of a bone defect, various bone grafts have been widely implanted into defect sites. However, several drawbacks, such as immunologic rejection, insufficient tissue healing, or secondary damage to the donor site, are still concerns. These limitations of the current bone grafts can be overcome by use of a natural ECM that can influence basic cellular behaviors, such as proliferation, migration, and differentiation. Herein, we introduce a novel bone decellularized ECM (bdECM) material extracted from bovine bones via demineralization and decellularization. This sequential process enabled us to prepare raw material possessing higher contents of native BMP-2 and BMP-7, and a fibrous microarchitecture resembling the collagen bundle in the ECM was maintained. Furthermore, residual calcium and phosphate originated from the bovine bone were considerably removed, and contamination by residual DNA was significantly reduced during the fabrication process. These features enabled the scaffold successfully promote mineralization of the cultured primary osteoblasts in vitro, which was significantly better than those from the bone powder or demineralized bone matrix. In a mouse calvarial defect, new bone formation was improved in the bdECM group and the density of those was also higher than other groups.

中文翻译:

脱细胞骨细胞外基质的生物制备及应用在有效骨再生中的应用

摘要 为了促进骨缺损的有效愈合,各种骨移植物已被广泛植入缺损部位。然而,一些缺点,如免疫排斥、组织愈合不足或供体部位的继发性损伤,仍然令人担忧。当前骨移植物的这些局限性可以通过使用可以影响基本细胞行为(如增殖、迁移和分化)的天然 ECM 来克服。在此,我们介绍了一种通过脱矿和脱细胞从牛骨中提取的新型骨脱细胞 ECM (bdECM) 材料。这种连续的过程使我们能够制备具有更高含量的天然 BMP-2 和 BMP-7 的原材料,并保持了类似于 ECM 中胶原束的纤维微结构。此外,来自牛骨的残留钙和磷酸盐被显着去除,并且在制造过程中残留 DNA 的污染显着减少。这些特征使支架成功地促进体外培养的原代成骨细胞的矿化,这明显优于骨粉或脱矿骨基质的矿化。在小鼠颅骨缺损中,bdECM 组的新骨形成得到改善,并且其密度也高于其他组。这明显优于来自骨粉或脱矿骨基质的那些。在小鼠颅骨缺损中,bdECM 组的新骨形成得到改善,并且其密度也高于其他组。这明显优于来自骨粉或脱矿骨基质的那些。在小鼠颅骨缺损中,bdECM 组的新骨形成得到改善,并且其密度也高于其他组。
更新日期:2020-03-01
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