Surgery, radiation, chemotherapy and immunotherapy are the most common brain cancer treatment options determined by the type and the stage of the illness. Monitoring the effect of radiotherapy becomes as important as a cancer diagnosis. Within this context, the main goal of this study was to provide new insights into the monitoring of biochemical changes of medulloblastoma exposed to gamma irradiation with a low dose of 2 and 10 Gy using a label-free method (Raman microspectroscopy) and compare it with labelled techniques (metabolic activity and oxidative stress assays). Results showed that Raman spectroscopy can correctly classify the response of medulloblastoma cells to gamma irradiation with a specificity and sensitivity of 80.35% and 79.65%, respectively. Collected Raman spectra display measurable radiobiological effects that correlated with the dose of 2 and 10 Gy according to conducted multivariate statistical analysis. A CellROX Orange fluorescence probe was able to monitor the oxidative stress in live medulloblastoma cells post-irradiation.

中文翻译：

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通过拉曼光谱法监测治疗剂量的γ射线对髓母细胞瘤的影响

手术，放射线，化学疗法和免疫疗法是最常见的脑癌治疗选择，取决于疾病的类型和阶段。监测放射治疗的效果与癌症诊断同样重要。在此背景下，本研究的主要目的是使用无标记方法（拉曼光谱法）对低剂量的2和10 Gyγ射线照射的髓母细胞瘤的生化变化的监测提供新的见解，并将其与标记技术（代谢活性和氧化应激测定）。结果表明，拉曼光谱能正确分类髓母细胞瘤细胞对γ射线的反应，其特异性和敏感性分别为80.35％和79.65％。根据进行的多元统计分析，收集的拉曼光谱显示出可测量的放射生物学效应，其与2和10 Gy的剂量相关。CellROX Orange荧光探针能够监测放射后活髓母细胞瘤细胞中的氧化应激。