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Two combinatorial patterns of telomere histone marks in plants with canonical and non-canonical telomere repeats.
The Plant Journal ( IF 7.2 ) Pub Date : 2020-01-13 , DOI: 10.1111/tpj.14653
Kateřina Adamusová 1, 2 , Solmaz Khosravi 3 , Satoru Fujimoto 4 , Andreas Houben 3 , Sachihiro Matsunaga 4 , Jiří Fajkus 1, 2 , Miloslava Fojtová 1, 2
Affiliation  

Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are crucial for the maintenance of genome integrity. In most plants, telomeres consist of conserved tandem repeat units comprising the TTTAGGG motif. Recently, non-canonical telomeres were described in several plants and plant taxons, including the carnivorous plant Genlisea hispidula (TTCAGG/TTTCAGG), the genus Cestrum (Solanaceae; TTTTTTAGGG), and plants from the Asparagales order with either a vertebrate-type telomere repeat TTAGGG or Allium genus-specific CTCGGTTATGGG repeat. We analyzed epigenetic modifications of telomeric histones in plants with canonical and non-canonical telomeres, and further in telomeric chromatin captured from leaves of Nicotiana benthamiana transiently transformed by telomere CRISPR-dCas9-eGFP, and of Arabidopsis thaliana stably transformed with TALE_telo C-3×GFP. Two combinatorial patterns of telomeric histone modifications were identified: (i) an Arabidopsis-like pattern (A. thaliana, G. hispidula, Genlisea nigrocaulis, Allium cepa, Narcissus pseudonarcissus, Petunia hybrida, Solanum tuberosum, Solanum lycopersicum) with telomeric histones decorated predominantly by H3K9me2; (ii) a tobacco-like pattern (Nicotiana tabacum, N. benthamiana, C. elegans) with a strong H3K27me3 signal. Our data suggest that epigenetic modifications of plant telomere-associated histones are related neither to the sequence of the telomere motif nor to the lengths of the telomeres. Nor the phylogenetic position of the species plays the role; representatives of the Solanaceae family are included in both groups. As both patterns of histone marks are compatible with fully functional telomeres in respective plants, we conclude that the described specific differences in histone marks are not critical for telomere functions.

中文翻译:

具有典型和非典型端粒重复序列的植物中端粒组蛋白标记的两种组合模式。

端粒是线性真核染色体末端的核蛋白结构,对维持基因组完整性至关重要。在大多数植物中,端粒由包含TTTAGGG基序的保守串联重复单元组成。最近,在几种植物和植物分类中描述了非规范的端粒,包括肉食性植物Genlisea hispidula(TTCAGG / TTTCAGG),Cestrum属(茄科; TTTTTTGGG)和来自芦笋的带有脊椎动物型端粒重复序列的植物。 TTAGGG或葱属属特异性CTCGGTTATGGG重复。我们分析了具有典型和非典型端粒的植物中端粒组蛋白的表观遗传修饰,并进一步分析了从由端粒CRISPR-dCas9-eGFP瞬时转化的本氏烟草叶片捕获的端粒染色质,用TALE_telo C-3×GFP稳定转化的拟南芥。鉴定出端粒组蛋白修饰的两种组合模式:(i)拟南芥样模式(拟南芥,组球藻,黑刺李,葱属洋葱,水仙假水仙,矮牵牛,马铃薯,马铃薯,番茄)以端粒组蛋白为主修饰。通过H3K9me2; (ii)具有强烈H3K27me3信号的烟草样图案(烟草,本氏烟草,秀丽隐杆线虫)。我们的数据表明,植物端粒相关组蛋白的表观遗传修饰既不涉及端粒基序的序列,也不涉及端粒的长度。物种的系统发育位置也不起作用。茄科的代表都包括在这两个群体中。
更新日期:2019-12-13
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