当前位置: X-MOL 学术J. Mater. Sci. Mater. Med. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A novel bilayer zein/MMT nanocomposite incorporated with H. perforatum oil for wound healing.
Journal of Materials Science: Materials in Medicine ( IF 3.7 ) Pub Date : 2019-12-14 , DOI: 10.1007/s10856-019-6332-9
Seda Gunes 1 , Sedef Tamburaci 1 , Funda Tihminlioglu 2
Affiliation  

Recently, layered structures composed of nanofibers have gained attention as a novel material to mimic skin tissue in wound healing applications. The aim of this study is to develop a novel hybrid bilayer material composed of zein based composite film and nanofiber layers as a wound dressing material. The upper layer was composed of H. perforatum oil incorporated zein film including MMT and the bottom layer was comprised of 3D electrospun zein/MMT nanofibers to induce wound healing with the controlled release of H. perforatum oil. The bilayer composites were characterized in terms of mechanical test, WVP, water uptake and surface wettability. Antimicrobial activity of the wound dressings against microorganisms were investigated by disc diffusion method. In vitro cytotoxicity of monolayer film and bilayer structure was performed using WST-1 assay on HS2 keratinocyte and 3T3 cell lines. Results indicated that the prepared monolayer films showed appropriate mechanical and gas barrier properties and surface wettability for wound healing. Controlled release of H. perforatum oil was obtained from fabricated membranes up to 48 h. Bilayer membranes showed antimicrobial activity against E. coli, S. aureus, and C. albicans and did not show any toxic effect on NIH3T3 mouse fibroblast and HS2 keratinocyte cell lines. In vitro scratch assay results indicated that H. perforatum oil had a wound healing effect by inducing fibroblast migration. The proliferation study supported these results by increasing fibroblast proliferation on H. perforatum oil loaded bilayer membranes.

中文翻译:

新颖的双层玉米醇溶蛋白/ MMT纳米复合材料与贯叶连翘油混合,用于伤口愈合。

近来,由纳米纤维组成的层状结构作为在伤口愈合应用中模仿皮肤组织的新型材料而受到关注。这项研究的目的是开发一种新型的混合双层材料,该材料由玉米蛋白基复合膜和纳米纤维层组成,作为伤口包扎材料。上层由掺有贯叶连翘油的玉米蛋白膜组成,包括MMT,而下层由3D电纺玉米醇溶蛋白/ MMT纳米纤维组成,以诱导伤口愈合,并控制了贯叶连翘油的释放。根据机械测试,WVP,吸水率和表面润湿性对双层复合材料进行了表征。通过椎间盘扩散法研究了伤口敷料对微生物的抗菌活性。使用WST-1分析对HS2角质形成细胞和3T3细胞系进行单层膜和双层结构的体外细胞毒性试验。结果表明,所制备的单层膜表现出合适的机械和气体阻隔性能以及用于伤口愈合的表面润湿性。穿孔膜油的控释可在48小时内从人造膜中获得。双层膜对大肠杆菌,金黄色葡萄球菌和白色念珠菌具有抗菌活性,对NIH3T3小鼠成纤维细胞和HS2角质形成细胞系没有任何毒性作用。体外刮擦试验结果表明,贯叶连翘油通过诱导成纤维细胞迁移具有伤口愈合作用。增殖研究通过增加在贯叶连翘油上负载的双层膜上的成纤维细胞增殖来支持这些结果。结果表明,所制备的单层膜表现出合适的机械和气体阻隔性能以及用于伤口愈合的表面润湿性。穿孔膜油的控释可在48小时内从人造膜中获得。双层膜对大肠杆菌,金黄色葡萄球菌和白色念珠菌具有抗菌活性,对NIH3T3小鼠成纤维细胞和HS2角质形成细胞系没有任何毒性作用。体外刮擦试验结果表明,贯叶连翘油通过诱导成纤维细胞迁移具有伤口愈合作用。增殖研究通过增加在贯叶连翘油上负载的双层膜上的成纤维细胞增殖来支持这些结果。结果表明,所制备的单层膜表现出合适的机械和气体阻隔性能以及用于伤口愈合的表面润湿性。穿孔膜油的控释可在48小时内从人造膜中获得。双层膜对大肠杆菌,金黄色葡萄球菌和白色念珠菌具有抗菌活性,对NIH3T3小鼠成纤维细胞和HS2角质形成细胞系没有任何毒性作用。体外刮擦试验结果表明,贯叶连翘油通过诱导成纤维细胞迁移具有伤口愈合作用。增殖研究通过增加穿有贯叶连翘油的双层膜上的成纤维细胞增殖来支持这些结果。穿孔膜油的控释可在48小时内从人造膜中获得。双层膜对大肠杆菌,金黄色葡萄球菌和白色念珠菌具有抗菌活性,对NIH3T3小鼠成纤维细胞和HS2角质形成细胞系没有任何毒性作用。体外刮擦试验结果表明,贯叶连翘油通过诱导成纤维细胞迁移具有伤口愈合作用。增殖研究通过增加在贯叶连翘油上负载的双层膜上的成纤维细胞增殖来支持这些结果。穿孔膜油的控释可在48小时内从人造膜中获得。双层膜对大肠杆菌,金黄色葡萄球菌和白色念珠菌具有抗菌活性,对NIH3T3小鼠成纤维细胞和HS2角质形成细胞系没有任何毒性作用。体外刮擦试验结果表明,贯叶连翘油通过诱导成纤维细胞迁移具有伤口愈合作用。增殖研究通过增加在贯叶连翘油上负载的双层膜上的成纤维细胞增殖来支持这些结果。贯叶连翘油通过诱导成纤维细胞迁移而具有伤口愈合作用。增殖研究通过增加穿有贯叶连翘油的双层膜上的成纤维细胞增殖来支持这些结果。贯叶连翘油通过诱导成纤维细胞迁移而具有伤口愈合作用。增殖研究通过增加在贯叶连翘油上负载的双层膜上的成纤维细胞增殖来支持这些结果。
更新日期:2019-12-14
down
wechat
bug