Small RNAs (sRNAs) play a wide range of important roles in plants, from maintaining genome stability and enhancing disease resistance to regulating developmental processes. Over the past decade, next-generation sequencing technologies have allowed us to explore the sRNA populations with unprecedented depth and accuracy. The community has accumulated a tremendous amount of sRNA sequencing (sRNA-seq) data from various genotypes, tissues, and treatments. However, it has become increasingly challenging to access these "big data" and extract useful information, particularly for researchers lacking sophisticated bioinformatics tools and expensive computational resources. Here, we constructed an online website, Arabidopsis Small RNA Database (ASRD, http://ipf.sustech.edu.cn/pub/asrd), that allows users to easily explore the information from publicly available Arabidopsis (Arabidopsis thaliana) sRNA libraries. Our database contains ∼2.3 billion sRNA reads, representing ∼250 million unique sequences from 2,024 sRNA-seq libraries. We downloaded the raw data for all libraries and reprocessed them with a unified pipeline so that the normalized abundance of any particular sRNA or the sum of abundances of sRNAs from a genic or transposable element region can be compared across all libraries. We also integrated an online Integrative Genomics Viewer browser into our Web site for convenient visualization. ASRD is a free, web-accessible, and user-friendly database that supports the direct query of over 2,000 Arabidopsis sRNA-seq libraries. We believe this resource will help plant researchers take advantage of the vast next-generation sequencing datasets available in the public domain.

中文翻译：

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用于探索2000多个拟南芥小RNA图书馆的在线数据库。

小RNA（sRNA）在植物中起着广泛的重要作用，从维持基因组稳定性和增强抗病性到调节发育过程。在过去的十年中，下一代测序技术使我们能够以前所未有的深度和准确性探索sRNA群体。社区已经积累了来自各种基因型，组织和治疗方法的大量sRNA测序（sRNA-seq）数据。但是，访问这些“大数据”并提取有用的信息变得越来越具有挑战性，特别是对于缺少复杂的生物信息学工具和昂贵的计算资源的研究人员而言。在这里，我们建立了一个在线网站，拟南芥小RNA数据库（ASRD，http：//ipf.sustech.edu.cn/pub/asrd），使用户可以轻松地从公开的拟南芥（Arabidopsis thaliana）sRNA库中探索信息。我们的数据库包含约23亿个sRNA读物，代表来自2,024个sRNA-seq文库的约2.5亿个独特序列。我们下载了所有库的原始数据，并使用统一的管道对其进行了重新处理，以便可以在所有库中比较任何特定sRNA的标准化丰度或来自基因或转座元件区域的sRNA的丰度之和。我们还将在线集成式基因组学查看器浏览器集成到我们的网站中，以方便可视化。ASRD是一个免费的，可通过网络访问且对用户友好的数据库，它支持直接查询2000多个拟南芥sRNA-seq库。