OBJECTIVE Many microRNAs (miRNAs) have been reported to be aberrantly expressed in Alzheimer's disease (AD) patients. The present study aimed to explore the diagnostic value and neuroprotective role of miR-193a-3p in AD. METHODS 108 sporadic AD patients and 93 healthy controls were included. An Aβ25-35 insult cellular AD model of PC12 and SH-SY5Y was established. The relative expression levels of miR-193a-3p were calculated using qRT-PCR. Receiver operating characteristic (ROC) curve was applied to evaluate the usefulness of miR-193a-3p for detecting AD. Cell viability and apoptotic rates were calculated. Luciferase reporter assay was performed to confirm the interaction between miR-193a-3p and PTEN. RESULTS miR-193a-3p expression was downregulated in both AD patients and the cellular AD model (all P < 0.001). Remarkable positive association was detected between serum miR-193a-3p level and MMSE score in AD patients (r = 0.5889, P < 0.0001). The diagnostic sensitivity and specificity were 89.8% and 77.4%, respectively, and the area under the curve (AUC) was 0.914. Overexpression of miR-193a-3p weakened Aβ25-35 induced cell viability inhibition, and reduced Aβ25-35 induced cell apoptosis in PC12 cells (all P < 0.01). Downregulation of miR-193a-3p intensified the effect of Aβ25-35 PTEN was proved to be the target gene of miR-193a-3p. CONCLUSION MiR-193a-3p could be a novel biomarker for AD diagnosis, and may protect against neurotoxicity in AD by targeting PTEN.

中文翻译：

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miR-193a-3p对阿尔茨海默氏病和miR-193a-3p的诊断价值通过靶向PTEN来减轻淀粉样β诱导的神经毒性。

目的据报道，许多微RNA（miRNA）在阿尔茨海默氏病（AD）患者中异常表达。本研究旨在探讨miR-193a-3p在AD中的诊断价值和神经保护作用。方法包括108例散发性AD患者和93例健康对照者。建立了PC12和SH-SY5Y的Aβ25-35侵袭性细胞AD模型。使用qRT-PCR计算miR-193a-3p的相对表达水平。接收器工作特性（ROC）曲线用于评估miR-193a-3p对检测AD的有用性。计算细胞活力和凋亡率。进行荧光素酶报告基因测定以证实miR-193a-3p和PTEN之间的相互作用。结果AD患者和细胞AD模型中miR-193a-3p表达均下调（所有P <0.001）。在AD患者中，血清miR-193a-3p水平与MMSE评分之间存在显着的正相关性（r = 0.5889，P <0.0001）。诊断灵敏度和特异性分别为89.8％和77.4％，曲线下面积（AUC）为0.914。miR-193a-3p的过表达减弱了PC12细胞中Aβ25-35诱导的细胞活力抑制，并降低了Aβ25-35诱导的细胞凋亡（所有P <0.01）。miR-193a-3p的下调增强了Aβ25-35PTEN的作用，被证明是miR-193a-3p的目标基因。结论MiR-193a-3p可能是AD诊断的新型生物标志物，可以通过靶向PTEN来预防AD的神经毒性。曲线下面积（AUC）为0.914。miR-193a-3p的过表达减弱了PC12细胞中Aβ25-35诱导的细胞活力抑制，并降低了Aβ25-35诱导的细胞凋亡（所有P <0.01）。miR-193a-3p的下调增强了Aβ25-35PTEN的作用，被证明是miR-193a-3p的目标基因。结论MiR-193a-3p可能是AD诊断的新型生物标志物，可以通过靶向PTEN来预防AD的神经毒性。曲线下面积（AUC）为0.914。miR-193a-3p的过表达减弱了PC12细胞中Aβ25-35诱导的细胞活力抑制，并降低了Aβ25-35诱导的细胞凋亡（所有P <0.01）。miR-193a-3p的下调增强了Aβ25-35PTEN的作用，被证明是miR-193a-3p的目标基因。结论MiR-193a-3p可能是AD诊断的新型生物标志物，可以通过靶向PTEN来预防AD的神经毒性。