The aim of this study was to compare the ability of endogenous Lactobacillus paracasei ATR6 to grow in unsupplemented bovine cheese whey (CW) and ricotta whey (RW), and to evaluate the efficiency of these media as encapsulating agents for the spray-drying (SD) of this bacterium. Growth assays indicated that both CW and RW are adequate culture media since L. paracasei ATR6 grew satisfactorily (~ 4 log cycles) in both compared to the control medium (skimmed milk). Concerning their potential as encapsulating agents, results showed that survival rates after SD were similar for CW and RW (> 78%), as were the yields (> 47%) of this process. CW allowed the formation of smaller capsules (5.31 ± 1.27 μm) than RW (15.21 ± 7.24 μm), with lower moisture: 8.60% and 13.52%, respectively. Powders were stored at 25 °C for 60 days, during which CW and RW maintained satisfactory bacterial viability. Water activity increased, yet remained below 0.3. Moisture content of CW and RW powders was relatively stable throughout the 60 days; however, values obtained in this study are considered to lie outside the adequate range (4–7%) for product stability. We did not observe color changes in CW and RW microcapsules during storage. Due to its lower protein content, RW offered less protection, which led to higher loss in acidification ability of the strain. CW and RW are suitable culture media and encapsulating agents for the growth of L. paracasei ATR6; however, the former offered better protection, which enabled the maintenance of biological activity.

中文翻译：

---

干酪乳清和乳清干酪乳清用于内源性乳酸菌的生长和包封

这项研究的目的是比较内源性副干酪乳杆菌ATR6在未补充牛奶酪乳清（CW）和乳清干酪乳清（RW）中的生长能力，并评估这些介质作为喷雾干燥包封剂的效率（SD） ）的细菌。生长测定表明，自副干酪乳杆菌以来，顺时针和逆时针都是适当的培养基与对照培养基（脱脂牛奶）相比，两者的ATR6均令人满意地生长（〜4个对数周期）。关于它们作为包封剂的潜力，结果表明，SD后的CW和RW存活率相似（> 78％），该过程的收率也相似（> 47％）。与RW（15.21±7.24μm）相比，CW可以形成较小的胶囊（5.31±1.27μm），且水分较低：分别为8.60％和13.52％。粉末在25°C下储存60天，在此期间CW和RW保持令人满意的细菌生存力。水分活度增加，但仍低于0.3。在整个60天内，CW和RW粉末的水分含量相对稳定；但是，该研究中获得的值被认为超出了产品稳定性的适当范围（4–7％）。在储存过程中，我们没有观察到CW和RW微胶囊的颜色变化。由于其蛋白质含量较低，RW提供的保护较少，从而导致菌株酸化能力的损失更大。CW和RW是合适的培养基和包封剂，用于生长副干酪乳杆菌ATR6；但是，前者提供了更好的保护，从而可以维持生物活性。