Due to the significant growth of food production, the potential likelihood of food contamination is increasing. Foodborne illness caused by bacterial pathogens has considerably increased over the past decades, while at the same time, the species of harmful microorganisms also varied. Conventional bacterial culturing methods have been unable to satisfy the growing requirement for food safety inspections and food quality assurance. Therefore, rapid and simple detection methods are urgently needed. The loop-mediated isothermal amplification (LAMP) technology is a highly promising approach for the rapid and sensitive detection of pathogens, which allows nucleic acid amplification under isothermal conditions. The integration of the LAMP assay onto a microfluidic chip is highly compatible with point-of-care or resource-limited settings, as it offers the capability to perform experiments in combination with high screening efficiency. Here, we provide an overview of recent advances in LAMP-based microfluidic chip technology for detecting pathogens, based on real-time or endpoint determination mechanisms. We also discuss the promoting aspects of using the LAMP technique in a microfluidic platform, to supply a guideline for further molecular diagnosis and genetic analysis.

中文翻译：

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基于环介导的等温扩增的微流控芯片，用于病原体检测。

由于粮食生产的显着增长，潜在的粮食污染可能性正在增加。在过去的几十年中，由细菌病原体引起的食源性疾病已大大增加，与此同时，有害微生物的种类也有所不同。传统的细菌培养方法已经不能满足食品安全检查和食品质量保证日益增长的要求。因此，迫切需要快速且简单的检测方法。环路介导的等温扩增（LAMP）技术是用于病原体快速灵敏检测的极有前途的方法，它允许在等温条件下进行核酸扩增。LAMP测定法与微流控芯片的集成与现场护理或资源有限的设置高度兼容，因为它提供了结合高筛选效率进行实验的功能。在这里，我们基于实时或端点确定机制，概述了基于LAMP的微流控芯片技术在检测病原体方面的最新进展。我们还讨论了在微流体平台上使用LAMP技术的促进方面，为进一步的分子诊断和遗传分析提供指导。