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A kinase-dependent checkpoint prevents escape of immature ribosomes into the translating pool.
PLOS Biology ( IF 9.8 ) Pub Date : 2019-12-13 , DOI: 10.1371/journal.pbio.3000329
Melissa D Parker 1 , Jason C Collins 1 , Boguslawa Korona 1 , Homa Ghalei 1 , Katrin Karbstein 1, 2
Affiliation  

Premature release of nascent ribosomes into the translating pool must be prevented because these do not support viability and may be prone to mistakes. Here, we show that the kinase Rio1, the nuclease Nob1, and its binding partner Pno1 cooperate to establish a checkpoint that prevents the escape of immature ribosomes into polysomes. Nob1 blocks mRNA recruitment, and rRNA cleavage is required for its dissociation from nascent 40S subunits, thereby setting up a checkpoint for maturation. Rio1 releases Nob1 and Pno1 from pre-40S ribosomes to discharge nascent 40S into the translating pool. Weak-binding Nob1 and Pno1 mutants can bypass the requirement for Rio1, and Pno1 mutants rescue cell viability. In these strains, immature ribosomes escape into the translating pool, where they cause fidelity defects and perturb protein homeostasis. Thus, the Rio1-Nob1-Pno1 network establishes a checkpoint that safeguards against the release of immature ribosomes into the translating pool.

中文翻译:

激酶依赖性检查点可防止未成熟的核糖体逃逸到翻译池中。

必须防止新生核糖体过早释放到翻译池中,因为它们不支持生存能力并且可能容易出错。在这里,我们表明激酶 Rio1、核酸酶 Nob1 及其结合伙伴 Pno1 合作建立一个检查点,防止未成熟的核糖体逃逸到多核糖体中。Nob1 阻断 mRNA 募集,并且 rRNA 裂解是其从新生的 40S 亚基解离所必需的,从而为成熟建立了一个检查点。Rio1 从前 40S 核糖体释放 Nob1 和 Pno1,将新生的 40S 释放到翻译池中。弱结合的 Nob1 和 Pno1 突变体可以绕过对 Rio1 的要求,而 Pno1 突变体可以挽救细胞活力。在这些菌株中,未成熟的核糖体逃逸到翻译池中,在那里它们导致保真度缺陷并扰乱蛋白质稳态。因此,
更新日期:2019-12-17
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