BACKGROUND Human sperm cryopreservation is a simple and effective approach for male fertility preservation. METHODS To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation. RESULTS A total of 174 significantly differential proteins were identified between fresh and cryoperservated sperm: 98 proteins decreased and 76 proteins increased in the cryopreservation group. Bioinformatic analysis revealed that metabolic pathways play an important role in cryopreservation, including: propanoate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism. Four different proteins involved in glycolysis were identified by Western blotting: GPI, LDHB, ADH5, and PGAM1. CONCLUSIONS Our work will provide valuable information for future investigations and pathological studies involving sperm cryopreservation.

中文翻译：

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人类精子冷冻保存的定量蛋白质组学表征：使用独立于数据的采集质谱。

背景技术人类精子冷冻保存是一种用于男性生育力保存的简单有效的方法。方法为了确定这一过程中潜在的蛋白质组学变化，使用了具有高定量准确性和高可复制性蛋白质组学技术的数据独立采集（DIA）技术来定量表征人类精子冷冻保存的蛋白质组学。结果新鲜和冷冻精子之间总共鉴定出174种差异显着的蛋白质：冷冻保存组中减少了98种蛋白质，增加了76种蛋白质。生物信息学分析表明，代谢途径在冷冻保存中起着重要作用，包括：丙酸酯代谢，乙醛酸和二羧酸代谢，糖酵解/糖异生和丙酮酸代谢。通过蛋白质印迹法鉴定了参与糖酵解的四种不同蛋白质：GPI，LDHB，ADH5和PGAM1。结论我们的工作将为精子冷冻保存的未来研究和病理学研究提供有价值的信息。