Lung adenocarcinoma associated transcript 1 (LUADT1) has been reported as an oncogenic long non-coding RNA (lncRNA) in lung adenocarcinoma, while its roles in small cell lung cancer (SCLC) are unknown. Our RNA interaction bioinformatics prediction showed that LUADT1 could form strong base pairing with miR-15a-3p, which is a tumor-suppressive miRNA that can target Twist1. We found that LUADT1 and Twist1 were upregulated in SCLC, while miR-15a-3p was downregulated in SCLC. However, LUADT1 was posively correlated with Twist1 but was not significnatly correlated with miR-15a-3p. Overexpression experiments showed that and LUADT1 and miR-15a-3p did not significantly affect the expression of each other. Moreover, LUADT1 overexpression mediated the upregualtion of Twist1, and miR-15a-3p overexpression played an oppsoite role. Transwell assays showed that LUADT1 and Twist1 overexpression mediated the increased rate of cell invasion and migration, while miR-15a-3p overexpression mediated the decreased rate of cell invasion and migration. In addition, miR-15a-3p overexpression played an oppsoite role and attenuated the effects of LUADT1 overexpression. Therefore, LUADT1 may sponge miR-15a-3p to upregulate Twist1 in SCLC, thereby promoting cancer cell invasion and migration. TRIAL REGISTRATION: 2017GZH-1-201,746,382, registered at Jan 02,2017.

中文翻译：

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LncRNA LUADT1使miR-15a-3p海绵化，从而在小细胞肺癌中上调Twist1。

肺腺癌相关转录本1（LUADT1）已被报道为肺腺癌中的致癌性长非编码RNA（lncRNA），但其在小细胞肺癌（SCLC）中的作用尚不清楚。我们的RNA相互作用生物信息学预测表明，LUADT1可以与miR-15a-3p形成强碱基配对，miR-15a-3p是一种靶向Twist1的肿瘤抑制性miRNA。我们发现，LUADT1和Twist1在SCLC中上调，而miR-15a-3p在SCLC中下调。但是，LUADT1与Twist1呈正相关，但与miR-15a-3p无显着关联。过表达实验表明，LUADT1和miR-15a-3p不会显着影响彼此的表达。此外，LUADT1的过表达介导了Twist1的上调，而miR-15a-3p的过表达起了鸦片作用。Transwell分析表明，LUADT1和Twist1过表达介导了细胞侵袭和迁移的速率增加，而miR-15a-3p过表达则介导了细胞侵袭和迁移的速率降低。此外，miR-15a-3p的过表达发挥了鸦片作用，并减弱了LUADT1过表达的作用。因此，LUADT1可能使miR-15a-3p上调SCLC中的Twist1，从而促进癌细胞的侵袭和迁移。试用注册：2017GZH-1-201,746,382，注册日期为2017年1月2日。LUADT1可能使miR-15a-3p上调SCLC中的Twist1，从而促进癌细胞的侵袭和迁移。试用注册：2017GZH-1-201,746,382，注册日期为2017年1月2日。LUADT1可能使miR-15a-3p上调SCLC中的Twist1，从而促进癌细胞的侵袭和迁移。试用注册：2017GZH-1-201,746,382，注册日期为2017年1月2日。