BACKGROUND Floppy eyelid syndrome is a disorder in which the tarsal plate is easily distensible and is currently treated with conservative or surgical measures. Human tarsal plate contains type I collagen, which is crosslinked in corneal tissue as a treatment for keratoconus. We hypothesized that collagen crosslinking would similarly stiffen tarsal plate tissue and investigated this in porcine and human tarsal plate specimens. METHODS Riboflavin-sensitized porcine and human tarsus samples were irradiated with ultraviolet-A light. Porcine experiments were analyzed with gross photographs, anterior segment optical computed tomography (AS-OCT) imaging, and tensile testing. A prospective study of human tarsus was performed on samples from patients undergoing wedge resection for floppy eyelid syndrome and was analyzed with AS-OCT and tensile testing. RESULTS 73 porcine adnexa and 9 patients (16 eyelids) who underwent wedge excision were included in the study. Grossly, greater stiffness was observed in crosslinked porcine tissue. AS-OCT imaging in porcine tissue showed a distinct hyperreflective band in crosslinked specimens whose area and intensity increased with longer treatment time (P = 0.003); this band was also visible in crosslinked human specimens. Tensile testing was performed, but results were not statistically significant. CONCLUSIONS AS-OCT imaging, which has not been previously described for tarsal plate, showed a characteristic change in crosslinked porcine and human specimens. Tissue stiffness was increased grossly, but changes in tensile properties were not statistically significant. Further study is warranted to determine relevance as a potential treatment for floppy eyelid syndrome.

中文翻译：

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胶原交联对猪和人human板的影响。

背景技术眼睑软盘综合征是一种疾病，其中睑板容易扩张并且目前通过保守或外科措施进行治疗。人tar板含有I型胶原蛋白，该蛋白在角膜组织中交联以治疗圆锥角膜。我们假设胶原蛋白交联会类似地使板组织变硬，并在猪和人的plate板样本中对此进行了研究。方法用核黄素敏化的猪和人骨样品用紫外线-A辐照。用大照片，前段光学计算机断层扫描（AS-OCT）成像和拉伸测试对猪实验进行了分析。对来自接受楔形切除术治疗的眼睑综合征的患者的样本进行的人的前瞻性研究，并通过AS-OCT和拉伸测试进行了分析。结果本研究包括73头猪附件和9例行楔形切除术的患者（16个眼睑）。总体上，在交联的猪组织中观察到更大的刚度。猪组织中的AS-OCT成像在交联的标本中显示出明显的高反射带，其面积和强度随着处理时间的延长而增加（P = 0.003）；在交联的人类标本中也可以看到该条带。进行了拉伸测试，但是结果没有统计学意义。结论AS-OCT成像以前没有描述过板，但在交联的猪和人标本中显示出特征性变化。组织刚度明显增加，但拉伸性能的变化在统计学上不显着。