Three-dimensional (3D) scaffolds with tailored stiffness, porosity, and conductive properties are particularly important in tissue engineering for electroactive cell attachment, proliferation, and vascularization. Carbon nanotubes (CNTs) and poly(3,4-ethylenedioxythiophene) (PEDOT) have been extensively used separately as neural interfaces showing excellent results. Herein, we combine both the materials and manufacture 3D structures composed exclusively of PEDOT and CNTs using a methodology based on vapor phase polymerization of PEDOT onto a CNT/sucrose template. Such a strategy presents versatility to produce porous scaffolds, after leaching out the sucrose grains, with different ratios of polymer/CNTs, and controllable and tunable electrical and mechanical properties. The resulting 3D structures show Young’s modulus typical of soft materials (20–50 kPa), as well as high electrical conductivity, which may play an important role in electroactive cell growth. The conductive PEDOT/CNT porous scaffolds present high biocompatibility after 3 and 6 days of C8-D1A astrocyte incubation.

中文翻译：

---

基于气相聚合的量身定制方法学，用于组织工程的PEDOT / CNT支架的制造

具有量身定制的刚度，孔隙率和导电特性的三维（3D）支架在组织工程中对电活性细胞的附着，增殖和血管形成特别重要。碳纳米管（CNTs）和聚（3,4-乙撑二氧噻吩）（PEDOT）已被广泛地单独用作神经界面，显示出优异的结果。在这里，我们将材料结合起来，并使用基于PEDOT气相聚合到CNT /蔗糖模板上的方法，制造仅由PEDOT和CNT组成的3D结构。在浸出蔗糖颗粒后，这种策略具有生产多孔支架的多功能性，所述支架具有不同的聚合物/ CNT比率，并且具有可控和可调节的电学和机械性能。生成的3D结构显示出软材料（20–50 kPa）的典型杨氏模量以及高电导率，这可能在电活性细胞生长中起重要作用。导电的PEDOT / CNT多孔支架在C8-D1A星形胶质细胞培养3天和6天后呈现出高生物相容性。