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Utilisation of Rhododendron luteum Sweet bioactive compounds as valuable source of enzymes inhibitors, antioxidant, and anticancer agents.
Food and Chemical Toxicology ( IF 4.3 ) Pub Date : 2019-12-16 , DOI: 10.1016/j.fct.2019.111052
Mohamad Fawzi Mahomoodally 1 , Elwira Sieniawska 2 , Kouadio Ibrahime Sinan 3 , Marie Carene Nancy Picot-Allain 1 , Serife Yerlikaya 4 , Mehmet Cengiz Baloglu 5 , Yasemin Celik Altunoglu 4 , Ismail Senkardes 6 , Kannan Rr Rengasamy 7 , Gokhan Zengin 3
Affiliation  

Ethnobotanical evidences report the use of Rhododendron luteum Sweet (Ericaceae) in traditional medicinal systems. However, R. luteum has been associated to the occurrence of 'mad honey' poisoning. In the present study, the ethyl acetate, methanol, and water extracts of R. luteum were investigated for their in vitro antioxidant, enzyme inhibition, and cytotoxic properties. The cytotoxicity of R. luteum extracts on A549 lung cancer cell line was evaluated using MTT cell viability assay. Besides, HPLC-ESI-MSn approach was employed to elucidate the secondary metabolite profiles of R. luteum in order to establish any structure-activity relationship. Methanol and water extracts of R. luteum possessed highest radical scavenging and reducing properties while the ethyl acetate extract showed highest metal chelating properties. In terms of enzyme inhibition, the methanol and ethyl acetate extracts of R. luteum, possessing epigallocatechin, were active inhibitors of cholinesterase enzymes, α-glucosidase, and tyrosinase. Water extract caused growth inhibition of A549 cells with 207.2 μg/ml IC50 value. Though R. luteum has received little scientific attention due to the occurrence of grayanotoxins in the plant, however, data presented in this work shows promising biological activity of R. luteum and highlighted its role as a potential source of antioxidant and key enzyme inhibitors.

中文翻译:

黄花杜鹃的甜味生物活性化合物可作为酶抑制剂,抗氧化剂和抗癌剂的重要来源。

人类植物学证据报道了在传统医学系统中使用了甜杜鹃(Ericaceae)。然而,黄腐霉菌与“疯蜂蜜”中毒的发生有关。在本研究中,研究了黄褐毛虫的乙酸乙酯,甲醇和水提取物的体外抗氧化剂,酶抑制作用和细胞毒性。黄体提取物对A549肺癌细胞系的细胞毒性使用MTT细胞活力分析进行评估。此外,采用HPLC-ESI-MSn方法阐明了黄斑病菌的次生代谢产物谱,以建立任何构效关系。黄萎病菌的甲醇和水提取物具有最高的自由基清除和还原性能,而乙酸乙酯提取物则具有最高的金属螯合性能。就酶的抑制作用而言,具有表没食子儿茶素的黄萎病菌的甲醇和乙酸乙酯提取物是胆碱酯酶,α-葡萄糖苷酶和酪氨酸酶的活性抑制剂。水提取物以207.2μg/ ml的IC50值引起A549细胞的生长抑制。尽管由于植物中灰质毒素的存在,导致黄褐斑病很少受到科学关注,但是,这项工作中提供的数据显示出黄褐斑病的有希望的生物学活性,并突出了其作为抗氧化剂和关键酶抑制剂的潜在来源的作用。
更新日期:2019-12-17
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