Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi-piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi-pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi-pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi-pre-piRISC resist leaving Yb bodies until Armi binds Piwi-pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi-pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi-piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.

中文翻译：

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Armitage 确定 Piwi-piRISC 处理从前体形成和质量控制到细胞器间易位的过程。

Piwi 和 piRNA 形成 piRNA 诱导的沉默复合物 (piRISC) 以抑制转座子。在当前模型中，Armitage (Armi) 将 Piwi-piRISC 前体 (pre-piRISC) 带到线粒体，在那里发生西葫芦依赖性 piRISC 成熟。在这里，我们表明 Armi 对于 Yb 小体中 Piwi-pre-piRISC 的形成是必需的，并且 Armi 触发 Piwi-pre-piRISC 从 Yb 小体中退出并易位到线粒体。Piwi-pre-piRISC 会抵抗离开 Yb 体，直到 Armi 通过 piRNA 前体结合 Piwi-pre-piRISC。Armi N 末端的缺失也阻止了 Piwi-pre-piRISC 从 Yb 体的退出。因此，Armi 以多层方式确定 Piwi-piRISC 处理，从前体形成和质量控制到细胞器间易位以实现成熟。