Pulsed arterial spin labelling remains a non-invasive and highly used method for the study of rodent cerebral blood flow (CBF). Flow-sensitive alternating inversion recovery (FAIR) is one of the most commonly used MR-sequences for this purpose and exists with many different strategies to record the images. This study investigates Look-Locker (LL) TrueFISP readout for FAIR as an alternative to the standard EPI readout, which is provided by the manufacturer. The aim was to show the improved image quality using TrueFISP and to verify the reproducibility of the determination of the cerebral blood flow values. The measurement of many inversion points also allowed to investigate the influence of the correct blood relaxation rate on the fit of the CBF data. For the LL-FAIR TrueFISP an in-house written method was created. The method was tested on a group of C57BL/6 mice at the field strength of 9.4 T. The results show CBF maps with less distortion than for EPI and the values found are in good agreement with the literature. A comparison of the CBF values found with EPI and LL-TrueFISP shows very small differences, most being not significant. In conclusion, the method presented gives equivalent CBF maps in comparison to standard FAIR-EPI. Both methods have the same measurement time. TrueFISP has the advantage to EPI of producing undistorted images over larger areas of the mouse brain. It is advisable to check the value of the blood relaxation rate by measurement or to estimate it as a fitting parameter.

中文翻译：

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Look-Locker FAIR TrueFISP，可在9.4 T的鼠标上进行动脉自旋标记。

脉冲动脉自旋标记法仍然是研究啮齿动物脑血流（CBF）的一种非侵入性和高度使用的方法。流量敏感交替反转恢复（FAIR）是为此目的最常用的MR序列之一，并且以多种不同的策略来记录图像。这项研究调查了FAIR的Look-Locker（LL）TrueFISP读数，以替代制造商提供的标准EPI读数。目的是显示使用TrueFISP改善的图像质量，并验证确定脑血流值的可重复性。许多反转点的测量还允许研究正确的血液松弛率对CBF数据拟合的影响。对于LL-FAIR TrueFISP，创建了内部书面方法。该方法在一组C57BL / 6小鼠的场强为9.4 T的条件下进行了测试。结果显示，与EPI相比，CBF图具有较小的失真，并且发现的值与文献相符。与EPI和LL-TrueFISP发现的CBF值的比较显示出很小的差异，大多数差异不明显。总之，与标准FAIR-EPI相比，本文提出的方法可提供等效的CBF图。两种方法具有相同的测量时间。TrueFISP具有EPI的优势，可以在较大的鼠标大脑区域上生成不失真的图像。建议通过测量检查血液松弛率的值或将其估计为合适的参数。结果表明，与EPI相比，CBF映射的失真较小，并且找到的值与文献非常吻合。与EPI和LL-TrueFISP发现的CBF值的比较显示出很小的差异，大多数差异不明显。总之，与标准FAIR-EPI相比，本文提出的方法可提供等效的CBF图。两种方法具有相同的测量时间。TrueFISP具有EPI的优势，可以在较大的鼠标大脑区域上生成不失真的图像。建议通过测量检查血液松弛率的值或将其估计为合适的参数。结果表明，与EPI相比，CBF映射的失真较小，并且找到的值与文献非常吻合。与EPI和LL-TrueFISP发现的CBF值的比较显示出很小的差异，大部分差异不显着。总之，与标准FAIR-EPI相比，本文提出的方法可提供等效的CBF图。两种方法具有相同的测量时间。TrueFISP具有EPI的优势，可以在较大的鼠标大脑区域上生成不失真的图像。建议通过测量检查血液松弛率的值或将其估计为合适的参数。两种方法具有相同的测量时间。TrueFISP具有EPI的优势，可以在较大的鼠标大脑区域上生成不失真的图像。建议通过测量检查血液松弛率的值或将其估计为合适的参数。两种方法具有相同的测量时间。TrueFISP具有EPI的优势，可以在较大的鼠标大脑区域上生成不失真的图像。建议通过测量检查血液松弛率的值或将其估计为合适的参数。