The present study aimed to evaluate the quality of fresh sushi in Egypt. Fifty samples of sushi (Salmo salar) were collected from restaurants in Alexandria, Egypt. Paraffin, semi-thin and ultra-thin sections were used for parasitological analysis bylight and transmission electron microscopy. Bacteria were isolated by the dilution plate and direct plate methods and identified by a Vitek system. Twenty (40%) of the total examined samples showed microsporidia and helminth metacercariae infections. Histochemical stains showed distinct pinkish-red pyriform microspores embedded in muscular tissue stained with Gram, periodic acid-Schiff (PAS), and Ziehl–Neelsen (ZN) stains. Semi-thin sections showed double membrane xenoma-inducing granulomas containing spores at different developmental stages. Empty sporophorous vesicles and free spores were observed in the electron microscopic images. A bacteriological assay showed forty samples (80%) contaminated with human pathogenic bacteria with the average total bacterial counts ranging from 32 to 526 CFU/g. Four species of human pathogenic bacteria were identified in the examined samples, namelyKlebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, andSerratia plymuthicain 40, 38, 11, and 6 samples, respectively. These constitute the first record of fresh sushi product in Egypt and indicate the potential pathogenicity associated with raw seafood products.

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埃及新鲜寿司、鲑鱼寄生菌和细菌的评价和微量分析

本研究旨在评估埃及新鲜寿司的质量。五十个寿司样品（鲑鱼) 是从埃及亚历山大的餐馆收集的。石蜡、半薄和超薄切片用于寄生虫学分析l光学和透射电子显微镜。通过稀释板法和直接板法分离细菌，并通过 Vitek 系统进行鉴定。20 (40%) 的总检查样本显示微孢子虫和蠕虫囊蚴感染。组织化学染色显示嵌入肌肉组织中的明显的粉红色梨状小孢子，用革兰氏、高碘酸-希夫 (PAS) 和 Ziehl-Neelsen (ZN) 染色剂染色。半薄切片显示含有不同发育阶段孢子的双膜异种瘤诱导肉芽肿。在电子显微镜图像中观察到空的孢子囊泡和游离孢子。细菌学检测显示 40 个样本 (80%) 被人类致病菌污染，平均细菌总数为 32 至 526 CFU/g。肺炎克雷伯菌、大肠杆菌、金黄色葡萄球菌， 和沙雷氏菌分别在 40、38、11 和 6 个样本中。这些构成了埃及新鲜寿司产品的第一个记录，并表明与生海鲜产品相关的潜在致病性。