当前位置: X-MOL 学术Analyst › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A pro-gastrin-releasing peptide imprinted photoelectrochemical sensor based on the in situ growth of gold nanoparticles on a MoS2 nanosheet surface.
Analyst ( IF 4.2 ) Pub Date : 2020-01-08 , DOI: 10.1039/c9an02201e
Xing Wang 1 , Hongping Deng , Chen Wang , Qiuxi Wei , Yanying Wang , Xiaoxing Xiong , Chunya Li , Wenwen Li
Affiliation  

Lamellar MoS2 nanosheets were successfully prepared by hydrothermal synthesis using 1-(3-mercaptopropyl)-3-methyl-imidazolium bromine (MIMBr) ionic liquid as a sulfur source and a morphology control agent, and sodium molybdate as a molybdenum source. Gold nanoparticles were assembled on the surface of MoS2 nanosheets by the in situ reduction of chloroauric acid at low temperatures to successfully fabricate AuNP/2D-MoS2 nanocomposites, thus improving photoelectrochemical response. AuNP/2D-MoS2 nanocomposites were used as photoelectrically active materials modified onto a glassy carbon electrode surface to construct a photoelectrochemical (PEC) sensor. Then, using 1-(N-pyrrolpropyl)-3-methyl-imidazolium bromine (PMIMBr) ionic liquid as a functional monomer and pro-gastrin-releasing peptide (Pro-GRP) as a template, a molecularly imprinted polymerized ionic liquid film was electrochemically deposited on an AuNP/2D-MoS2/GCE surface. Upon removing the templates, a molecularly imprinted photoelectrochemical sensor was constructed for the sensing of a tumor marker, pro-gastrin-releasing peptide. Experimental conditions including ascorbic acid concentration, polymerization conditions, incubation time, and pH value of the incubation solution have been optimized. Under the optimized conditions, the molecularly imprinted photoelectrochemical sensor can specifically detect the target protein Pro-GRP in the range of 0.02 ng mL-1-5 ng mL-1 with a detection limit of 0.0032 ng mL-1 (S/N = 3). The practicability of this photoelectrochemical sensor was demonstrated by accurately determining Pro-GRP in human serum samples.

中文翻译:

基于金纳米颗粒在MoS2纳米片表面上原位生长的促胃泌素释放肽印迹光电化学传感器。

通过使用1-(3-巯基丙基)-3-甲基咪唑溴(MIMBr)离子液体作为硫源和形态控制剂,以及钼酸钠作为钼源通过水热合成成功制备了片状MoS2纳米片。通过在低温下原位还原氯金酸将金纳米颗粒组装在MoS2纳米片的表面上,从而成功制备AuNP / 2D-MoS2纳米复合材料,从而改善了光电化学反应。AuNP / 2D-MoS2纳米复合材料用作修饰到玻璃碳电极表面的光电活性材料,以构建光电化学(PEC)传感器。然后,以1-(N-吡咯丙基)-3-甲基咪唑溴(PMIMBr)离子液体为功能单体,并以胃泌素释放肽(Pro-GRP)为模板,将分子印迹聚合的离子液体膜电化学沉积在AuNP / 2D-MoS2 / GCE表面。去除模板后,构建了分子印迹光电化学传感器,用于感测肿瘤标志物,胃泌素释放肽。优化了包括抗坏血酸浓度,聚合条件,孵育时间和孵育溶液的pH值在内的实验条件。在优化的条件下,分子印迹光电化学传感器可以特异性检测目标蛋白Pro-GRP,范围为0.02 ng mL-1-5 ng mL-1,检测极限为0.0032 ng mL-1(S / N = 3 )。通过准确测定人血清样品中的Pro-GRP,证明了这种光电化学传感器的实用性。
更新日期:2020-02-17
down
wechat
bug