We use quantitative phase digital holographic microscopy (QP-DHM) to image and quantify the beating movement of cardiomyocytes, derived from induced pluripotent stem cells (iPSCs), in control and drug-treated conditions. The development of an analysis algorithm has allowed extracting from the recorded quantitative phase signal (QPS) a set of several parameters that can efficiently characterize the cardiomyocytes beating patterns. Based on this approach, we monitored the effects of E-4031 (a class III antiarrhythmic drug) and isoprenaline (a common medication for bradycardia and heart block problems) on the cardiomyocyte beating patterns. Our results show that some effects specific to the mode of action of the drugs used can be identified. This stresses that QP-DHM can represent a promising label-free approach to identify new drug candidates by measuring their effects on iPSC-derived cardiomyocytes.

中文翻译：

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用数字全息成像对​​药物处理过的心肌细胞进行无标记自动定量

我们使用定量相数字全息显微镜（QP-DHM）来成像和量化在控制和药物治疗条件下源自诱导多能干细胞（iPSC）的心肌细胞的跳动运动。分析算法的发展已允许从记录的定量相位信号（QPS）中提取一组可以有效表征心肌细胞跳动模式的参数。基于这种方法，我们监测了E-4031（III类抗心律不齐药物）和异丙肾上腺素（一种用于心动过缓和心脏传导阻滞的常用药物）对心肌细胞跳动模式的影响。我们的结果表明，可以确定某些与所用药物的作用方式有关的作用。