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Controlled deprotection and release of a small molecule from a compartmented synthetic tissue module
Communications Chemistry ( IF 5.9 ) Pub Date : 2019-12-12 , DOI: 10.1038/s42004-019-0244-y
Michael J. Booth , Idil Cazimoglu , Hagan Bayley

Synthetic tissues built from communicating aqueous droplets offer potential applications in biotechnology, however, controlled release of their contents has not been achieved. Here we construct two-droplet synthetic tissue modules that function in an aqueous environment. One droplet contains a cell-free protein synthesis system and a prodrug-activating enzyme and the other a small-molecule prodrug analog. When a Zn2+-sensitive protein pore is made in the first droplet, it allows the prodrug to migrate from the second droplet and become activated by the enzyme. With Zn2+ in the external medium, the activated molecule is retained in the module until it is released on-demand by a divalent cation chelator. The module is constructed in such a manner that one or more, potentially with different properties, might be incorporated into extended synthetic tissues, including patterned materials generated by 3D-printing. Such modules will thereby increase the sophistication of synthetic tissues for applications including controlled multidrug delivery.



中文翻译:

控制小分子从隔离的​​合成组织模块中的脱保护和释放

由连通的水滴构建的合成组织在生物技术中具有潜在的应用,但是,其内容物的受控释放尚未实现。在这里,我们构建了在水环境中起作用的两滴合成组织模块。一个小滴包含无细胞的蛋白质合成系统和前药激活酶,另一个小分子包含小分子前药类似物。当在第一个液滴中形成Zn 2+敏感蛋白孔时,它可使前药从第二个液滴中迁移出来并被酶激活。含锌2+在外部介质中,被激活的分子被保留在模块中,直到被二价阳离子螯合剂按需释放。该模块以一种或多种可能具有不同特性的方式构建,可以并入扩展的合成组织中,包括通过3D打印生成的图案化材料。这样的模块将因此增加用于包括受控多药递送的应用的合成组织的复杂性。

更新日期:2019-12-13
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