The dysferlin membrane repair complex contains a small complex, S100A10-annexin A2, which initiates membrane repair by recruiting the protein AHNAK to the membrane, where it interacts via binding sites in the C-terminal region. However, no molecular data are available for the membrane binding of the various proteins involved in this complex. Therefore, the present study investigated the membrane binding of AHNAK to elucidate its role in the cell membrane repair process. A chemically synthesized peptide (pAHNAK), comprising the 20 amino acids in the C-terminal domain of AHNAK, was applied to Langmuir monolayer models, and the binding parameters and insertion angles were measured with surface tensiometry and ellipsometry. The interaction of pAHNAK with lipid bilayers was studied using 31P solid-state nuclear magnetic resonance. pAHNAK preferentially and strongly interacted with phospholipids that comprised negatively charged polar head groups with unsaturated lipids. This finding provides a better understanding of AHNAK membrane behavior and the parameters that influence its function in membrane repair.

中文翻译：

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AHNAK的残基5654-5673与磷脂单层和双层之间的AHNAK C末端肽膜结合相互作用。

dysferlin膜修复复合物包含一个小的复合物S100A10-annexin A2，该复合物通过将AHNAK蛋白募集到膜来启动膜修复，在膜中它通过C端区域中的结合位点相互作用。然而，没有分子数据可用于该复合物中涉及的各种蛋白质的膜结合。因此，本研究调查了AHNAK的膜结合，以阐明其在细胞膜修复过程中的作用。将化学合成的肽（pAHNAK）包含在AHNAK的C端结构域中，包含20个氨基酸，并将其应用于Langmuir单层模型，并通过表面张力和椭圆偏振法测量结合参数和插入角度。使用31 P固态核磁共振研究了pAHNAK与脂质双层的相互作用。pAHNAK与包含不饱和脂质带负电荷的极性头基的磷脂优先且强烈地相互作用。该发现提供了对AHNAK膜行为和影响其在膜修复中功能的参数的更好理解。