A comprehensive characterization of the molecular processes controlling cell fate decisions is essential to derive stable progenitors and terminally differentiated cells that are functional from human pluripotent stem cells (hPSCs). Here, we report the use of quantitative proteomics to describe early proteome adaptations during hPSC differentiation toward pancreatic progenitors. We report that the use of unbiased quantitative proteomics allows the simultaneous profiling of numerous proteins at multiple time points, and is a valuable tool to guide the discovery of signaling events and molecular signatures underlying cellular differentiation. We also monitored the activity level of pathways whose roles are pivotal in the early pancreas differentiation, including the Hippo signaling pathway. The quantitative proteomics data set provides insights into the dynamics of the global proteome during the transition of hPSCs from a pluripotent state toward pancreatic differentiation.

中文翻译：

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人多能干细胞衍生胰腺祖细胞的动态蛋白质组分析

控制细胞命运决定的分子过程的综合表征对于从人类多能干细胞 (hPSC) 中获得具有功能的稳定祖细胞和终末分化细胞至关重要。在这里，我们报告使用定量蛋白质组学来描述 hPSC 向胰腺祖细胞分化过程中的早期蛋白质组适应。我们报告说，使用无偏定量蛋白质组学可以在多个时间点同时分析多种蛋白质，并且是指导发现信号事件和细胞分化背后的分子特征的宝贵工具。我们还监测了在早期胰腺分化中起关键作用的通路的活性水平，包括 Hippo 信号通路。