BACKGROUND Pseudomonas aeruginosa is a nosocomial pathogen that causes severe infections in immunocompromised patients. Biofilm plays a significant role in the resistance of this bacterium and complicates the treatment of its infections. In this study, the effect of lyticase and β-glucosidase enzymes on the degradation of biofilms of P. aeruginosa strains isolated from cystic fibrosis and burn wound infections were assessed. Moreover, the decrease of ceftazidime minimum biofilm eliminating concentrations (MBEC) after enzymatic treatment was evaluated. RESULTS This study demonstrated the effectiveness of both enzymes in degrading the biofilms of P. aeruginosa. In contrast to the lyticase enzyme, β-glucosidase reduced the ceftazidime MBECs significantly (P < 0.05). Both enzymes had no cytotoxic effect on the A-549 human lung carcinoma epithelial cell lines and A-431 human epidermoid carcinoma cell lines. CONCLUSION Considering the characteristics of the β-glucosidase enzyme, which includes the notable degradation of P. aeruginosa biofilms and a significant decrease in the ceftazidime MBECs and non-toxicity for eukaryotic cells, this enzyme can be a promising therapeutic candidate for degradation of biofilms in burn wound patients, but further studies are needed.

中文翻译：

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裂解酶和β-葡萄糖苷酶对具有不同基因谱的铜绿假单胞菌菌株生物膜降解的功效。

背景技术铜绿假单胞菌是在免疫受损的患者中引起严重感染的医院病原体。生物膜在该细菌的抗性中起重要作用，并使感染的治疗变得复杂。在这项研究中，评估了裂解酶和β-葡萄糖苷酶对从囊性纤维化和烧伤创面感染分离的铜绿假单胞菌菌株生物膜降解的影响。此外，评估了酶处理后头孢他啶最小生物膜消除浓度（MBEC）的降低。结果这项研究证明了这两种酶在降解铜绿假单胞菌生物膜方面的有效性。与裂解酶相反，β-葡萄糖苷酶显着降低了头孢他啶MBEC（P <0.05）。两种酶对A-549人肺癌上皮细胞系和A-431人表皮样癌细胞系均无细胞毒性作用。结论考虑到β-葡糖苷酶的特性，包括铜绿假单胞菌生物膜的显着降解和头孢他啶MBEC的显着减少以及对真核细胞的无毒性，该酶可作为一种有希望的治疗候选生物膜降解的生物膜。烧伤伤口的患者，但需要进一步研究。