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Stability of milk and gliadin on swabs during 7 days under different storage conditions
Food Control ( IF 6 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.foodcont.2019.107054
Virginie Barrere , Jérémie Théolier , Sébastien Lacroix , Steven Zbylut , Alexcia Valdez , Nick Collopy , Brandon Lahey , Samuel Godefroy

Abstract Food industries rely on swabbing to control allergen and gluten occurrences in their manufacturing plant. However, collecting allergens from a surface with a swab and further analysis can be affected by numerous aspects, like the operator, the allergen, the swab fabric, the ELISA kit, and the procedure. In addition, food companies often send swabs to external laboratories, but there are no available data to describe the effect of time and temperature on the allergen or gluten stability on the swabs. This study presents the stability of milk and gliadin collected from a stainless-steel surface using different swab types (cotton, Dacron, rayon, polyester, and polyurethane). The protein concentrations were tested on days 0, 1, 2, 3, and 7 by an ELISA technique to follow the protein concentration over time. The swabs with gliadin were stored at 37 °C for 7 days to mimic poor storage conditions. The swabs used to collect milk were stored at 4 and 37 °C. The experiments indicated that, except for two cases, there was no difference in gliadin concentrations between day 1 and the other days; the same observation was made for milk. However, the swab types were not equivalent and some led to false-negative results. Validation of those observations was undertaken at a commercial laboratory. To reduce the risk of false-negatives and the effect of factors influencing the result (operator, swab, procedure, and allergen), it is recommended to swab an area in triplicate.

中文翻译:

不同储存条件下 7 天内牛奶和麦胶蛋白在拭子上的稳定性

摘要 食品工业依靠擦拭来控制制造工厂中过敏原和麸质的出现。但是,使用拭子从表面收集过敏原并进行进一步分析可能会受到许多方面的影响,例如操作员、过敏原、拭子织物、ELISA 试剂盒和程序。此外,食品公司经常将拭子送到外部实验室,但没有可用数据来描述时间和温度对拭子上过敏原或麸质稳定性的影响。本研究展示了使用不同拭子类型(棉、涤纶、人造丝、聚酯和聚氨酯)从不锈钢表面收集的牛奶和麦醇溶蛋白的稳定性。在第 0、1、2、3 和 7 天通过 ELISA 技术测试蛋白质浓度以跟踪蛋白质浓度随时间的变化。含有麦胶蛋白的拭子在 37 °C 下储存 7 天,以模拟不良的储存条件。用于收集牛奶的拭子分别储存在 4°C 和 37°C 下。实验表明,除两种情况外,第1天和其他天的麦醇溶蛋白浓度没有差异;对牛奶也进行了同样的观察。然而,拭子类型并不相同,有些会导致假阴性结果。这些观察结果的验证是在商业实验室进行的。为了降低假阴性的风险和影响结果的因素(操作员、拭子、程序和过敏原)的影响,建议对一个区域进行三次擦拭。第 1 天和其他天的麦醇溶蛋白浓度没有差异;对牛奶也进行了同样的观察。然而,拭子类型并不相同,有些会导致假阴性结果。这些观察结果的验证是在商业实验室进行的。为了降低假阴性的风险和影响结果的因素(操作员、拭子、程序和过敏原)的影响,建议对一个区域进行三次擦拭。第 1 天和其他天的麦醇溶蛋白浓度没有差异;对牛奶也进行了同样的观察。然而,拭子类型并不相同,有些会导致假阴性结果。这些观察结果的验证是在商业实验室进行的。为了降低假阴性的风险和影响结果的因素(操作员、拭子、程序和过敏原)的影响,建议对一个区域进行三次擦拭。
更新日期:2020-04-01
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