This study aimed to develop a chitosan‐based hydrogel containing a mixture of flavonoids isolated from the leaves of Passiflora edulis Sims and to evaluate its stability, antioxidant properties, and wound healing effects on cutaneous lesions in diabetic rats. in vitro studies were carried out to evaluate the biocompatibility and flavonoid release from the chitosan hydrogel. in vivo wound healing studies were conducted on male Wistar rats, where the injured tissue was removed for histological analysis and determination of lipid peroxidation, superoxide dismutase activity, and glutathione peroxidase activity. From the histological analysis and macroscopic evaluation of the contraction of the wounds, it was observed that the formulation presented wound healing properties. In addition, treatment of the wound with the formulation stimulated the antioxidant defense system, suggesting a beneficial effect during the treatment of skin lesions in diabetic rats, especially in the first few days after wounding. According to these results, we can conclude that the chitosan hydrogel containing the flavonoid analyzed in this study has potential use as dressings in the treatment of wounds.

中文翻译：

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开发含有从西番莲叶中提取的黄酮类化合物的壳聚糖水凝胶，并评价其用于治疗糖尿病小鼠皮肤损伤的抗氧化和伤口愈合特性。

本研究旨在开发一种基于壳聚糖的水凝胶，其中含有从西番莲叶子中分离的类黄酮混合物Sims 并评估其稳定性、抗氧化特性和伤口愈合对糖尿病大鼠皮肤病变的影响。进行了体外研究以评估壳聚糖水凝胶的生物相容性和类黄酮释放。对雄性 Wistar 大鼠进行了体内伤口愈合研究，其中取出受伤组织进行组织学分析和脂质过氧化、超氧化物歧化酶活性和谷胱甘肽过氧化物酶活性的测定。从伤口收缩的组织学分析和宏观评估，观察到该制剂呈现伤口愈合特性。此外，用该制剂治疗伤口刺激了抗氧化防御系统，表明在治疗糖尿病大鼠皮肤损伤期间具有有益作用，尤其是在受伤后的最初几天。根据这些结果，我们可以得出结论，本研究中分析的含有类黄酮的壳聚糖水凝胶具有作为伤口治疗敷料的潜在用途。