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3D-engineered GelMA conduit filled with ECM promotes regeneration of peripheral nerve.
Journal of Biomedical Materials Research Part A ( IF 4.9 ) Pub Date : 2019-12-16 , DOI: 10.1002/jbm.a.36859
Hui Gong 1 , Haosheng Fei 1 , Qifan Xu 1 , Maling Gou 2 , Harry H Chen 1, 2
Affiliation  

Autologous transplantation remains the golden standard for peripheral nerve repair. However, many drawbacks, such as the risk of reoperation or nerve injury remain associated with this method. To date, commercially available artificial nerve conduits comprise hollow tubes. By providing physical guiding and biological cues, tissue engineered conduits are promising for bridging peripheral nerve defects. The present study focuses on the preparation of artificial composite nerve conduits by 3D bio‐printing. 3D‐printed molds with a tubular cavity were filled with an Engelbreth‐Holm‐Swarm (EHS) Hydrogel mimicking the extracellular matrix (ECM) basement membrane. Chemically cross‐linked gelatin methacryloyl (GelMA) was used to form the conduit backbone, while EHS Hydrogels improved nerve fiber growth while shortening repair time. Statistical significant difference had been found between the blank conduit and the composite conduit group on compound muscle action potential after 4 months. On the other hand, results between the composite conduit group and the autograft group were of no statistical differences. All results above showed that the composite conduit filled with EHS Hydrogel can promote the repair of peripheral nerve and may become a promising way to treat peripheral nerve defects.

中文翻译:

充满 ECM 的 3D 工程 GelMA 导管促进周围神经再生。

自体移植仍然是周围神经修复的金标准。然而,许多缺点,例如再次手术或神经损伤的风险,仍然与这种方法有关。迄今为止,市售的人工神经导管包括空心管。通过提供物理引导和生物线索,组织工程导管有望弥合周围神经缺陷。本研究的重点是通过 3D 生物打印制备人工复合神经导管。具有管状腔的 3D 打印模具填充有模拟细胞外基质 (ECM) 基底膜的 Engelbreth-Holm-Swarm (EHS) 水凝胶。化学交联的明胶甲基丙烯酰 (GelMA) 用于形成导管骨架,而 EHS 水凝胶改善了神经纤维的生长,同时缩短了修复时间。4个月后,空白导管组与复合导管组复合肌动作电位差异有统计学意义。另一方面,复合导管组与自体移植组的结果无统计学差异。以上结果表明,填充EHS水凝胶的复合导管可以促进周围神经的修复,可能成为治疗周围神经缺损的一种有前景的方法。
更新日期:2019-12-16
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