The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV-Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via -NH2 group, while horizontal binding mode via S and -NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine.

中文翻译：

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绿色合成和无毒L-蛋氨酸封端的银和金纳米粒子的表征。

开发了一种简单的绿色方法，用于合成稳定的L-蛋氨酸（L-Met）封端的银（Ag @ LM NPs）和金（Au @ LM NPs）纳米颗粒（NPs），而无需添加任何其他还原剂或稳定剂。胶体分散体通过UV-Vis分光光度法表征。NP的大小和球形通过透射电子显微镜评估。它们的表面被原子力显微镜，傅立叶变换红外光谱，动态光散射和ζ电势测量所证实。密度泛函理论计算表明，L-Met在Ag和Au表面上的优先吸附模式是通过-NH2基团的垂直键合几何形状，而通过S和-NH2基团的水平键合模式也是可能的。NPs的遗传毒性（通过微核分析评估），以及它们对某些氧化应激参数（过氧化氢酶活性，丙二醛水平）的影响，均在体外以人外周血细胞为模型系统进行了评估。NP对原子力显微镜研究的淋巴细胞细胞形态的影响表明，NPs处理后细胞膜仍不受影响。当考虑NPs对过氧化氢酶活性和丙二醛水平的影响时，两种颗粒都不能促进氧化应激。然而，用Ag @ LM NPs处理淋巴细胞诱导了浓度依赖性的微核发生率增强和细胞增殖抑制，而Au @ LM NPs促进了细胞增殖，对微核形成没有显着影响。与Au @ LM NP相比，Ag @ LM NP更容易引起DNA损伤，