Keratin is a cytoskeletal scaffolding protein essential for wound healing and tissue recovery. The aim of the study was to evaluate the potential role of insoluble fur keratin-derived powder containing silver nanoparticles (FKDP-AgNP) in the allogenic full-thickness surgical skin wound model in diabetic mice. The scanning electron microscopy image evidenced that the keratin surface is covered by a single layer of silver nanoparticles. Data obtained from dynamic light scattering and micellar electrokinetic chromatography showed three fractions of silver nanoparticles with an average diameter of 130, 22.5, and 5 nm. Microbiologic results revealed that the designed insoluble FKDP-AgNP dressing to some extent inhibit the growth of Escherichia coli and Staphylococcus aureus. In vitro assays showed that the FKDP-AgNP dressing did not inhibit fibroblast growth or induce hemolysis. In vivo studies using a diabetic mice model confirmed biocompatible properties of the insoluble keratin dressings. FKDP-AgNP significantly accelerated wound closure and epithelization at Days 5 and 8 (p < .05) when compared with controls. Histological examination of the inflammatory response documented that FKDP-AgNP-treated wounds contained predominantly macrophages, whereas their untreated variants showed mixed cell infiltrates rich in neutrophils. Wound inflammatory response based on macrophages favors tissue remodeling and healing. In conclusion, the investigated FKDP-AgNP dressing consisting of an insoluble fraction of keratin, which is biocompatible, significantly accelerated wound healing in a diabetic mouse model.

中文翻译：

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评估含银纳米颗粒的角蛋白生物材料作为糖尿病小鼠全层皮肤伤口模型中潜在的伤口敷料。

角蛋白是对伤口愈合和组织恢复必不可少的细胞骨架支架蛋白。这项研究的目的是评估在糖尿病小鼠的同种异体全厚度外科手术皮肤伤口模型中，含不溶性毛皮角蛋白的含银纳米颗粒粉末（FKDP-AgNP）的潜在作用。扫描电子显微镜图像证明角蛋白表面被单层银纳米颗粒覆盖。从动态光散射和胶束电动色谱获得的数据表明，三部分银纳米颗粒的平均直径分别为130、22.5和5 nm。微生物学结果表明，设计的不溶性FKDP-AgNP敷料在一定程度上抑制了大肠杆菌和金黄色葡萄球菌的生长。体外测定表明，FKDP-AgNP敷料不抑制成纤维细胞生长或诱导溶血。使用糖尿病小鼠模型的体内研究证实了不溶性角蛋白敷料的生物相容性。与对照组相比，FKDP-AgNP在第5天和第8天显着加速了伤口闭合和上皮形成（p <.05）。炎症反应的组织学检查表明，用FKDP-AgNP处理的伤口主要含有巨噬细胞，而未经处理的变体显示混合细胞浸润液富含中性粒细胞。基于巨噬细胞的伤口炎症反应有利于组织重塑和愈合。总之，所研究的FKDP-AgNP敷料由不溶性角蛋白组成，具有生物相容性，可显着加速糖尿病小鼠模型的伤口愈合。