Derivatives (1–15) of steroidal and indole class were synthesized using different strategies. These compounds were characterized by ¹H NMR spectroscopy and EI-MS, respectively. The synthetic derivatives were examined for their cytotoxic effects on human adenocarcinoma cells (HCT-116) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and morphometric analysis. The cytotoxic effects of all the compounds were observed after 48 h treatment and it was found that out of fifteen, four compounds 1, 2, 3, and 14 showed inhibitory action on the cancer cells. We have calculated the IC₅₀ values for compounds 1, 2, 3, and 14 which were 22.50 µg/mL, 55.65 µg/mL, 21.35 µg/mL and 58.50 µg/mL, respectively. The compounds 3 (IC₅₀ = 21.35 µg/mL) and 1 (IC₅₀ = 22.50 µg/mL) showed highest inhibitory activities as compared to compounds 2 (IC₅₀ = 55.65 µg/mL) and 14 (IC₅₀ = 58.50 µg/mL). These results suggested that steroidal thiazole and indole derivatives are potent lead molecules having strong anti-cancer proliferative capabilities.

类固醇和吲哚类的衍生物（1 – 15）是使用不同的策略合成的。这些化合物分别通过¹ H NMR光谱和EI-MS进行表征。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）测定法和形态计量分析检查了合成衍生物对人腺癌细胞（HCT-116）的细胞毒性作用。48小时处理后，观察所有的化合物的细胞毒性作用，并且发现了这一点的15，四种化合物1，2，3，和14显示出对癌细胞的抑制作用。我们已经计算了化合物的IC ₅₀值1，2，3，和14，其分别为22.50微克/毫升，分别55.65微克/毫升，21.35微克/毫升和58.50微克/毫升。 与化合物2（IC ₅₀  = 55.65 µg / mL）和14（IC ₅₀  = 58.50μg / mL）相比，化合物3（IC ₅₀  = 21.35μg/ mL）和1（IC ₅₀ = 22.50μg/ mL）表现出最高的抑制活性。毫升）。这些结果表明，甾族噻唑和吲哚衍生物是具有强的抗癌增殖能力的有效的先导分子。